In vivo immunogenicity of bovine bone removed by a novel decellularization protocol based on supercritical carbon dioxide
In vivo immunogenicity of bovine bone removed by a novel decellularization protocol based on supercritical carbon dioxide
复制标题
基于超临界二氧化碳的新型脱细胞方案去除牛骨的体内免疫原性
DOI：
10.1080/21691401.2018.1457044
复制
发表时间：
2018-01-01
影响因子：
5.8
通讯作者：
Xu Bin
中科院分区：
文献类型：
--
作者：
Ling You;Xu Weikang;Xu Bin
创伤或感染相关的严重骨缺损导致全球医疗保健系统的巨大经济负担。组织工程学的最新进展为骨缺损的修复、替代和再生提供了新的策略，特别是在生物材料和异种组织脱细胞化方面。然而，骨结构和力学环境的复杂性限制了生物材料人工骨的合成。因此，我们的研究目的是开发一种具有良好免疫相容性的天然骨支架。结合超临界CO2脱细胞技术对牛骨进行脱细胞处理。为了研究小鼠对材料的免疫应答，对小鼠的组织学、脾脏指数、免疫细胞含量和体外增殖性能、细胞因子和免疫球蛋白轻链表达进行了表征。与新鲜骨组相比，脱细胞组的免疫反应明显降低。总之，通过该方法的脱细胞化可以获得具有良好免疫相容性的脱细胞支架。我们的研究结果表明，使用脱细胞BB作为骨生物工程支架的潜力。[图片]
Trauma or infections associated critical bone defects lead to a huge economic burden in the healthcare system worldwide. Recent advances in tissue engineering have led to potential new strategies for the repair, replacement, and regeneration of bone defects, especially in biomaterials and decellularization protocols from xenogenic tissues. However, the complexity in bone structure and mechanical environment limits the synthesis of artificial bone with biomaterials. Thus, the purpose of our study is to develop a natural bone scaffold with great immunocompatibility. We combined decellularization techniques base on SC-CO2 to decellularize bovine bone. In order to study the immune response of mice to materials, the histology, spleen index, immune cells contents and in vitro proliferative performance, cytokine and immunoglobulin light chain expression of mice were characterized. Compared with the fresh bone group, the immune responses of decellularized group were significantly reduced. In conclusion, decellularization via this method can achieve a decellularized scaffold with great immunocompatibility. Our findings suggest the potential of using decellularized BB as a scaffold for bone bioengineering.[GRAPHICS]