Comparison of the effects of three different combinations of general anesthetics on the electroretinogram of dogs
Comparison of the effects of three different combinations of general anesthetics on the electroretinogram of dogs
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DOI：
10.1007/s10633-009-9173-x
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发表时间：
2009-10-01
影响因子：
1.4
通讯作者：
Seo, Kang Moon
中科院分区：
文献类型：
--
作者：
Jeong, Man Bok;Narfstroem, Kristina;Seo, Kang Moon
本研究的目的是在相似的实验室条件下比较三种不同麻醉剂组合对相同动物视网膜电图的影响。使用犬标准方案，对12只健康小型雪纳瑞犬（MS）中的每一只使用硫喷妥钠-异氟烷（TI）、美托咪定-氯胺酮（MK）和甲苯噻嗪-氯胺酮（XK），后续麻醉方案之间间隔至少3周。暗视ERG包括暗适应后1、5、10、15和20 min的暗视低刺激强度（S）反应（分别命名为S1、S2、S3、S4和S5）和暗视标准刺激强度（S-ST）反应。明视ERG由明视单闪光（P）反应和31 Hz闪烁（P-FL）反应组成。对于S-ST（2.5 cd s/m2），TI法的a波振幅显著低于MK法（校正后P = 0.05）和XK法（校正后P = 0.03），a波的隐式时间显著短于MK法（校正后P = 0.04）。对于P（2.5 cd s/m（2）），使用XK的b波振幅显著高于使用MK（校正后P = 0.01）。TI的b波隐式时间在S1、S2和P-FL、S4和S-ST时分别显著长于MK，在S2和P-FL、S5和S-ST时分别显著短于XK。本研究的结果表明，TI的影响幅度和隐式时间的a波的S-ST和隐式时间的b波的情况下，使用XK或MK相对更大。因此，在临床研究中，似乎使用XK或MK比使用TI更有利。
The objective of this study is to compare the effects of three different anesthetic combinations on the electroretinogram in the same animals under similar laboratory conditions. Thiopental-isoflurane (TI), medetomidine-ketamine (MK), and xylazine-ketamine (XK) were used on each of 12 healthy miniature schnauzer dogs (MS) with a period of at least 3 weeks in between subsequent anesthesia protocols, using the Dog Standard Protocol. The scotopic ERGs consisted of scotopic low stimulus strength (S) responses designated S1, S2, S3, S4, and S5, at 1, 5, 10, 15, and 20 min after dark adaptation, respectively, and scotopic standard stimulus strength (S-ST) responses. The photopic ERGs consisted of a photopic single flash (P) response and 31 Hz flicker (P-FL) responses. For S-ST (2.5 cd s/m(2)), the amplitude of the a-wave using TI was significantly lower than that using MK (adjusted P = 0.05) and XK (adjusted P = 0.03), and the implicit time of the a-wave was significantly shorter than that using MK (adjusted P = 0.04). For P (2.5 cd s/m(2)), the amplitude of the b-wave using XK was significantly higher than that using MK (adjusted P = 0.01). The implicit times of the b-wave using TI was significantly longer and shorter than that of MK for S1, S2 and P-FL and for S4 and S-ST, respectively, and than that of XK for S2 and P-FL and for S5 and S-ST, respectively. The results of the present study showed that TI affected both the amplitude and the implicit time of the a-wave for S-ST and the implicit time of the b-wave relatively more so than was the case when using XK or MK. Therefore, it appears that either XK or MK could be advantageous to use rather than TI for clinical studies.