Tissue engineering of bone: an ectopic rat model.
Tissue engineering of bone: an ectopic rat model.
复制标题
DOI：
10.2741/s132
复制
发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Bahar, Hila
中科院分区：
文献类型：
--
作者：
Binderman, Itzhak;Yaffe, Avinoam;Bahar, Hila
组织工程学试图重现活组织的复杂性。为了测试各种支架或不断开发的细胞，我们在这里描述了一个模型，其中在DA大鼠皮下胸部部位的非骨环境中产生骨组织工程。在该模型中，在3-4周期间，细胞-基质相互作用可以模拟骨发育成组织良好的小骨样结构（包括新形成的骨髓）的正常级联。软骨、骨和骨髓的组织发生与成骨细胞分化所必需的早期转录调节因子的分子表达变化密切相关。我们测试了不同的有机，无机和聚合物支架及其与新鲜骨髓中存在的间充质干细胞的相互作用。在另一系列实验中，我们测试了从颅骨和骨膜培养物中分离的间充质群体在同一大鼠模型中形成骨的能力。可以得出结论，这种在体内模型是非常有效的研究细胞-支架相互作用影响的时间和空间的组织工程骨。
Tissue engineering is attempting to recreate the complexity of living tissues. In order to test a variety of scaffolds or cells that are constantly being developed, we describe here a model where tissue engineering of bone in a non-osseous environment at subcutaneous thoracic site of DA rats generates. In this model, cell - matix interactions can mimic the normal cascade of bone development into a well organized ossicle like structure including newly formed bone marrow, during 3-4 weeks. Histogenesis of cartilage, bone and bone marrow is closely related to changes in molecular expression of essential early transcriptional regulators of osteoblast differentiation. We tested different organic, anorganic and polymeric scaffolds and their interaction with mesenchymal stem cells present in fresh bone marrow. In another series of experiments we tested mesenchymal populations separated from cultures of calvaria and periosteum for their ability to form bone in the same rat model. It is concluded that this in vivo model is very potent in studying cell-scaffold interactions affecting the temporal and spatial tissue engineering of bone.