Telomere length and heredity:: Indications of paternal inheritance
Telomere length and heredity:: Indications of paternal inheritance
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DOI：
10.1073/pnas.0501724102
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发表时间：
2005-11-08
影响因子：
11.1
通讯作者：
Roos, G
中科院分区：
文献类型：
--
作者：
Nordfjäll, K;Larefalk, Å;Roos, G
细胞端粒长度与复制寿命有关。随着年龄的增长，端粒重复序列在体内外周血细胞中丢失，女性的端粒磨损比男性少。以往的研究表明，端粒长度和染色体特异性端粒长度模式部分是遗传的。遗传模式尚未澄清，但最近有人提出与X染色体有关。我们分析了外周血单个核细胞从49个无关的家庭端粒长度使用实时PCR方法。短期培养的EB病毒转化的淋巴母细胞从相同的个人（n = 130）进行了分析，以维持端粒长度和可能的性别连锁遗传的能力。结果发现，父亲-儿子（P = 0.011，n = 20）和父亲-女儿（P = 0.005，n = 22）之间的端粒长度存在显著相关性，而母亲-女儿（P = 0.463，n = 23）和母亲-儿子（P = 0.577，n = 18）之间的端粒长度无相关性。父-子相关显著（P < 0.0001），母-子相关显著（P = 0.361）。Epstein-Barr病毒培养物显示在大多数情况下端粒保存与初始单核细胞端粒长度呈负相关，其中短端粒显示最明显的延长。端粒长度是遗传的，并且获得了这种性状的父代-子代春季遗传的证据，而在体外端粒长度维持被发现依赖于初始端粒长度。
Cellular telomere length is linked to replicative life span. Telomere repeats are lost in peripheral blood cells in vivo by age, and women show less telomere attrition than men. Previous reports have indicated that telomere length and chromosome-specific telomerelength patterns partly are inherited. The mode of heredity has not been clarified, but a link to the X chromosome was recently suggested. We analyzed peripheral mononuclear cells from 49 unrelated families for telomere length using a real-time PCR method. Short-term cultured Epstein-Barr virus-transformed lymphoblasts from the same individuals (n = 130) were analyzed for ability to maintain telomere length and possible gender-linked inheritance. A statistically significant association between telomere lengths comparing father-son (P = 0.011, n = 20) and father-daughter (P = 0.005, n = 22) pairs was found. However, no correlation was observed between mother-daughter (P = 0.463, n = 23) or mother-son (P = 0.577, n = 18). The father-offspring correlation was highly significant (P < 0.0001), in contrast to mother-offspring (P = 0.361). Epstein-Barr virus cultures demonstrated in most cases telomere preservation inversely related to initial mononuclear cell telomere length with short telomeres displaying the most pronounced elongation. Telomere length is inherited, and evidence for a father-to-off spring heritage of this trait was obtained, whereas in vitro telomere length maintenance was found to be dependent on the initial telomere length.