Porcine stem cell factor/c-kit ligand: its molecular cloning and localization within the uterus.
Porcine stem cell factor/c-kit ligand: its molecular cloning and localization within the uterus.
复制标题
猪干细胞因子/c-kit配体：其分子克隆和子宫内定位。
作者：
Z. Zhang;R. Anthony
干细胞因子（SCF），或c-kit配体，是一种多能生长因子，在动物发育的各个方面都有重要作用，包括维持原始生殖细胞的活力。以猪子宫内膜mRNA为模板，采用RT-PCR方法扩增了猪SCF（pSCF）cDNA，并测定了其核苷酸序列。pSCF cDNA全长952 bp，含有一个开放阅读框，编码274个氨基酸。推导的pSCF的氨基酸序列分别与人、大鼠和小鼠SCF的氨基酸序列约86%、83%和82%相同，并且含有4个保守的半胱氨酸残基和Asn连接的糖基化位点。在pSCF中鉴定了一个额外的氨基酸，Glu 130，其不在人（hSCF）、大鼠（rSCF）或小鼠（mSCF）序列中。从第16天妊娠子宫内膜获得的poly（A）+ RNA的北方分析显示约6.5 kb的转录物。该转录本的大小与全长SCF mRNA的大小一致，并且通过子宫内膜和卵巢总细胞RNA（tcRNA）的RT-PCR/Southern杂交分析未检测到选择性剪接的pSCF mRNA的出现。应用原位杂交技术对猪子宫内膜间质组织中SCF mRNA进行了定位。妊娠似乎不是子宫内膜组织中pSCF mRNA表达的先决条件，因为在妊娠和非妊娠母猪的组织和tcRNA中均可检测到。子宫pSCF表达的生物学意义目前尚不清楚，但它可能参与子宫内的细胞内通讯。
Stem cell factor (SCF), or c-kit ligand, is a multipotent growth factor that has been implicated in an important role in various aspects of animal development, including maintenance of the viability of primordial germ cells. A porcine SCF (pSCF) cDNA was generated from porcine uterine endometrial mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR), and its nucleotide sequence was determined. The 952-bp pSCF cDNA contained an open reading frame encoding 274 amino acids. The deduced amino acid sequence of pSCF is approximately 86%, 83%, and 82%, identical to human, rat, and mouse SCFs, respectively; and it contains the four conserved cysteine residues and Asn-linked glycosylation sites. One additional amino acid was identified in pSCF, Glu130, which is not in the human (hSCF), rat (rSCF), or mouse (mSCF) sequences. Northern analysis of poly(A)+ RNA obtained from Day 16 pregnant endometrium revealed a transcript of approximately 6.5 kb. The size of this transcript is consistent with the size of full-length SCF mRNA, and the occurrence of alternatively spliced pSCF mRNAs were not detected by RT-PCR/Southern hybridization analysis of endometrial and ovarian total cellular RNA (tcRNA). Porcine SCF mRNA has been localized by in situ hybridization in porcine endometrial stromal tissue. Pregnancy does not appear to be a prerequisite for pSCF mRNA expression in endometrial tissue since it was detectable in tissue and tcRNA obtained from pregnant and nonpregnant gilts. The biological significance of uterine pSCF expression is currently unclear, but it probably participates in intracellular communication within the uterus.
植入前后大鼠和小鼠子宫中多肽生长因子合成和生长因子相关基因表达的调节。
DOI：
10.1530/jrf.0.0880721
发表时间：
1990
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Pollard,JW
通讯作者：
Pollard,JW