Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase.

Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase.
复制标题

DOI：
10.1126/science.2646717
复制
发表时间：
1989-03
期刊：
Science
影响因子：
56.9
通讯作者：
J. Sampson;A. Direnzo;L. Behlen;O. Uhlenbeck
J. Sampson;A. Direnzo;L. Behlen;O. Uhlenbeck
中科院分区：
综合性期刊1区
文献类型：
--
作者：
J. Sampson;A. Direnzo;L. Behlen;O. Uhlenbeck

文献摘要

被引文献

相似文献

未修饰的酵母tRNAPhe突变体的氨酰化动力学的分析表明，5个单链核苷酸是重要的酵母苯丙氨酰-tRNA合成酶的识别，只要它们被正确地定位在一个正确折叠的tRNA结构。当其他四种tRNA被改变为具有这五种核苷酸时，它们成为酶的接近正常的底物。
An analysis of the aminoacylation kinetics of unmodified yeast tRNAPhe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase, provided they were positioned correctly in a properly folded tRNA structure. When four other tRNAs were changed to have these five nucleotides, they became near-normal substrates for the enzyme.