Cloning and characterization of an esophageal-gland-specific chorismate mutase from the phytoparasitic nematode Meloidogyne javanica
Cloning and characterization of an esophageal-gland-specific chorismate mutase from the phytoparasitic nematode Meloidogyne javanica
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DOI：
10.1094/mpmi.1999.12.4.328
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发表时间：
1999-04-01
影响因子：
3.5
通讯作者：
Sussex, IM
中科院分区：
文献类型：
--
作者：
Lambert, KN;Allen, KD;Sussex, IM
根结线虫是专性植物寄生虫，通过诱导形成巨大的饲养细胞来改变植物细胞的生长和发育。据认为，线虫在进食时将其食道腺的分泌物注入植物细胞，这些分泌物导致巨细胞形成。为了阐明巨细胞形成的机制，本研究从爪哇根结线虫（Meloidogyne javanica）中克隆了食管腺基因。一个克隆，显示在线虫的食道腺中表达，编码潜在分泌的分支酸酯酶（CM），Chi是莽草酸途径中的关键分支点调节酶，并将分支酸酯转化为预苯酸酯，预苯酸酯是苯丙氨酸和酪氨酸的前体。莽草酸途径在动物中没有发现，但在植物中发现，在那里它产生芳香族氨基酸和衍生化合物，在生长和防御中发挥关键作用，因此，我们假设这种CM参与了线虫寄生植物的过程。
Root-knot nematodes are obligate plant parasites that alter plant cell growth and development by inducing the formation of giant feeder cells. It is thought that nematodes inject secretions from their esophageal glands into plant cells while feeding, and that these secretions cause giant cell formation. To elucidate the mechanisms underlying the formation of giant cells, a strategy was developed to clone esophageal gland genes from the root-knot nematode Meloidogyne javanica. One clone, shown to be expressed in the nematode's esophageal gland, codes for a potentially secreted chorismate mutase (CM), Chi is a key branch-point regulatory enzyme in the shikimate pathway and converts chorismate to prephenate, a precursor of phenylalanine and tyrosine, The shikimate pathway is not found in animals, but in plants, where it produces aromatic amino acids and derivative compounds that play critical roles in growth and defense, Therefore, we hypothesize that this CM is involved in allowing nematodes to parasitize plants.