Voltage sensitive phosphoinositide phosphatases of Xenopus: their tissue distribution and voltage dependence.
Voltage sensitive phosphoinositide phosphatases of Xenopus: their tissue distribution and voltage dependence.
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DOI：
10.1002/jcp.22854
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发表时间：
2011-11
影响因子：
5.6
通讯作者：
Jaffe, Laurinda A.
中科院分区：
文献类型：
--
作者：
Ratzan, William J.;Evsikov, Alexei V.;Okamura, Yasushi;Jaffe, Laurinda A.
电压敏感性磷酸酶（VSPs）是一种独特的蛋白质，其中膜电位控制酶的活性。它们由离子通道的电压传感器结构域组成，该离子通道与对磷酸肌醇特异性的脂质磷酸酶偶联，并且对于海鞘和斑马鱼VSP，已经发现磷酸酶活性通过膜去极化激活。这些蛋白质的生理功能尚不清楚，但它们在睾丸和胚胎中的表达表明在受精或发育中起作用。在这里，我们调查的表达模式和电压依赖性的VSP在两种青蛙，非洲爪蟾和非洲爪蟾热带，非常适合这些可能的功能的实验研究。X. laevis具有两个VSP基因（X1-VSP 1和X1-VSP 2），而X. tropicalis只有一个基因（Xt-VSP）。这些基因在睾丸、卵巢、肝脏和肾脏中的表达最高。我们的研究结果表明，虽然X1-VSP 2仅在生理范围外的正膜电位下激活，但X1-VSP 1和Xt-VSP磷酸酶活性在受精时调节精卵融合的电压范围内受到调节。
Voltage sensitive phosphatases (VSPs) are unique proteins in which membrane potential controls enzyme activity. They are comprised of the voltage sensor domain of an ion channel coupled to a lipid phosphatase specific for phosphoinositides, and for ascidian and zebrafish VSPs, the phosphatase activity has been found to be activated by membrane depolarization. The physiological functions of these proteins are unknown, but their expression in testis and embryos suggests a role in fertilization or development. Here we investigate the expression pattern and voltage dependence of VSPs in two frog species, Xenopus laevis and Xenopus tropicalis, that are well suited for experimental studies of these possible functions. X. laevis has two VSP genes (Xl-VSP1 and Xl-VSP2), whereas X. tropicalis has only one gene (Xt-VSP). The highest expression of these genes was observed in testis, ovary, liver, and kidney. Our results show that while Xl-VSP2 activates only at positive membrane potentials outside of the physiological range, Xl-VSP1 and Xt-VSP phosphatase activity is regulated in the voltage range that regulates sperm-egg fusion at fertilization.
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影响因子：
5.3
作者：
Chen, HM;Rossier, C;Antonarakis, SE
通讯作者：
Antonarakis, SE
影响因子：
3.7
作者：
Dimitrov D;He Y;Mutoh H;Baker BJ;Cohen L;Akemann W;Knöpfel T
通讯作者：
Knöpfel T
影响因子：
5.5
作者：
Murata, Yoshimichi;Okamura, Yasushi
通讯作者：
Okamura, Yasushi
影响因子：
64.8
作者：
JAFFE, LA
通讯作者：
JAFFE, LA
影响因子：
2.7
作者：
GREY, RD;BASTIANI, MJ;SCHERTEL, ER
通讯作者：
SCHERTEL, ER