Fos expression after mating in noradrenergic cells of the A1 and A2 areas of the medulla is altered by adrenalectomy.
Fos expression after mating in noradrenergic cells of the A1 and A2 areas of the medulla is altered by adrenalectomy.
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交配后髓质 A1 和 A2 区域去甲肾上腺素能细胞中的 Fos 表达因肾上腺切除术而改变。
DOI：
10.1111/j.1365-2826.2004.01231.x
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Erskine,MS
中科院分区：
文献类型：
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作者：
Cameron,NM;Ha,GK;Erskine,MS
在雌性大鼠中，腹侧去甲肾上腺素束（VNAB）的完整性对于将刺激从子宫颈和阴道传递到涉及交配诱导假妊娠的大脑区域是必要的。由于已知肾上腺激素会改变去甲肾上腺素能功能，因此我们检查了肾上腺切除术是否改变了通过 VNAB 投射的 A1 和 A2 去甲肾上腺素能细胞群中交配诱导的 Fos 表达。切除卵巢的雌性进行肾上腺切除术 (ADX) 或假手术 (Sham)，手术后 2 周给予雌激素和黄体酮并交配。他们从雄性那里接受了 15 次性交、5 次性交或 15 次无性交（仅坐骑）。交配后两小时，对大鼠进行灌注并收集大脑；对照组直接从笼子里取出后进行灌注。免疫细胞化学染色后，对 Fos 免疫反应性 (Fos-IR) 和多巴胺-β-羟化酶免疫反应性 (DBH-IR) 细胞以及用 Fos 标记的 DBH 细胞百分比 (% DBH/Fos) 进行计数。在 A1 区域，Fos-IR 和 DBH/Fos 百分比不受肾上腺切除术的影响。尽管发现交配处理对这两种措施都有总体影响，但没有特定的交配处理使标记细胞增加到高于家庭笼子水平。在尾部、中部和喙部 A2 中，15 次插入导致假手术雌性的 Fos-IR 显着增加（高于所有其他组），并且中部和喙部 A2 区域的 DBH/Fos 百分比更高。 ADX 雌性在 15 次交配后，Fos-IR 或 DBH/Fos 百分比没有增加。然而，在 A2 中部和头端，仅坐骑治疗后，ADX 雌性显示出 Fos-IR 和 DBH/Fos 百分比显着增加，高于仅坐骑治疗的雌性和所有其他 ADX 组。这些结果表明，肾上腺激素会抑制 A2 细胞对仅安装刺激的激活，但会促进 A2 细胞响应雄性的插入而激活。后一种效应可能是由于交配期间接受阴道宫颈刺激所产生的压力造成的。
In the female rat, the integrity of the ventral noradrenergic bundle (VNAB) is necessary to carry stimuli from the uterine cervix and vagina to brain areas involved in mating‐induced pseudopregnancy. Because adrenal hormones are known to alter noradrenergic function, we examined whether adrenalectomy altered mating‐induced Fos expression in the A1 and A2 noradrenergic cell groups that project through the VNAB. Ovariectomized females were adrenalectomized (ADX) or sham‐operated (Sham) and, 2 weeks after surgery, were given oestrogen and progesterone and mated. They received 15 intromissions, five intromissions or 15 mounts‐without‐intromission (mounts‐only) from a male. Two hours after mating, rats were perfused and brains were collected; controls were perfused after being taken directly from their home cage. After immunocytochemical staining, Fos‐immunoreactive (Fos‐IR) and dopamine‐β‐hydroxylase‐immunoreactive (DBH‐IR) cells and the percentage of DBH cells that were labelled with Fos (% DBH/Fos) were counted. In the A1 area, Fos‐IR and percentage DBH/Fos were not affected by adrenalectomy. Although an overall effect of mating treatment was found for both measures, no specific mating treatment increased labelled cells above home cage levels. In the caudal, middle and rostral A2, 15 intromissions induced a significant increase in Fos‐IR in Sham females above all other groups and a higher percentage of DBH/Fos in the middle and rostral A2 areas. ADX females showed no rise in either Fos‐IR or percentage DBH/Fos after 15 intromissions. However, in the middle and rostral A2, ADX females showed significantly increased Fos‐IR and percentage DBH/Fos after mounts‐only treatment above Sham mounts‐only females and all other ADX groups. These results demonstrate that adrenal hormones suppress activation of A2 cells to mounts‐only stimuli but contribute to A2 activation in response to intromissions from males. The latter effect may result from stress associated with receipt of vaginocervical stimulation during mating.