Studies on Interaction of New Medicine of Quinolone-Class with HSA and BSA by Using Fluorescence Enhancement and Fluorescence Quenching Theory
Studies on Interaction of New Medicine of Quinolone-Class with HSA and BSA by Using Fluorescence Enhancement and Fluorescence Quenching Theory
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发表时间：
2006
影响因子：
1
通讯作者：
Yang Man
中科院分区：
文献类型：
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作者：
Yang Man
利用荧光增强和荧光猝灭两种理论，研究和比较了四种喹诺酮类药物与人血清白蛋白（HSA）和牛血清白蛋白（BSA）的相互作用，并深入分析了药物与白蛋白的结合特性和常用的表征常数：解离常数、猝灭常数、猝灭效率、能量传递效率、供体与受体的距离。关于白蛋白与药物的结合类型，通过对4种药物与HSA和BSA的猝灭实验结果发现，这种由供体与受体结合引起的猝灭作用类型不是由生物大分子BSA自身决定的，而是由BSA与药物、供体与受体的分子结构及其相互匹配共同决定的。
By using the two theoriesfluorescence enhancement and fluorescence quenching,we studied and compared the inter action of four kinds of Quinolone-class-medicines with HSA and BSA,then analysed deeply the binding speciality of medicine and albumin and usual characterization constants: dissociation constant,quenching constant,quenching efficiency,energy-transfer efficiency,the distance between donor and accepter.About binding type of albumin with medicine,through the quenching experiment result of the four kinds of medicines with HSA and BSA,we found that this quenching action type caused by the binding of donor between acceptor was not determined by the oneself-biomacromolecule BSA,but together determined by the molecular structure of BSA and medicine,donor and acceptor,and their match with each other.