Contribution of the Peroxisomal acox Gene to the Dynamic Balance of Daumone Production in Caenorhabditis elegans
Contribution of the Peroxisomal acox Gene to the Dynamic Balance of Daumone Production in Caenorhabditis elegans
复制标题
DOI：
10.1074/jbc.m110.122663
复制
发表时间：
2010-09-17
影响因子：
4.8
通讯作者：
Paik, Young-Ki
中科院分区：
文献类型：
--
作者：
Joo, Hyoe-Jin;Kim, Kwang-Youl;Paik, Young-Ki
Dauer信息素或daumone是在不利的生长条件下中断发育和繁殖（Dauer幼虫）的信号分子，对于秀丽隐杆线虫中的细胞内稳态是必不可少的。根据早期的研究，在菌株N2中的dauer幼虫的形成是通过温度的升高而增强的，这表明参与dauer信息素生物合成或感测中的温度依赖性组分。几种天然存在的daumone类似物（e。G. daumone 1-3），并且预测这些分子在该线虫中在不同的生理环境中合成。为了阐明的分子调控系统，可能会影响动态平衡的特定的道蒙生产响应突然的温度变化，我们的特点是过氧化物酶体acox基因编码的酰基辅酶A氧化酶，这是预测催化的第一个反应，在生物合成的脂肪酸成分的道蒙。使用acox-1（ok 2257）突变体和一种新的，稳健的分析方法，我们定量了蠕虫体内三种最丰富的daumone，并表明acox可能有助于响应温度升高而动态产生不同数量的三种不同的daumone，这些变化在C.应对自然环境变化的能力。
Dauer pheromones or daumones, which are signaling molecules that interrupt development and reproduction (dauer larvae) during unfavorable growth conditions, are essential for cellular homeostasis in Caenorhabditis elegans. According to earlier studies, dauer larva formation in strain N2 is enhanced by a temperature increase, suggesting the involvement of a temperature-dependent component in dauer pheromone biosynthesis or sensing. Several naturally occurring daumone analogs (e. g. daumones 1-3) have been identified, and these molecules are predicted to be synthesized in different physiological settings in this nematode. To elucidate the molecular regulatory system that may influence the dynamic balance of specific daumone production in response to sudden temperature changes, we characterized the peroxisomal acox gene encoding acyl-CoA oxidase, which is predicted to catalyze the first reaction during biosynthesis of the fatty acid component of daumones. Using acox-1(ok2257) mutants and a new, robust analytical method, we quantified the three most abundant daumones in worm bodies and showed that acox likely contributes to the dynamic production of various quantities of three different daumones in response to temperature increase, changes that are critical in C. elegans for coping with the natural environmental changes it faces.