ANALYSIS OF CARDIOLIPIN MOLECULAR-SPECIES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF ITS DERIVATIVE 1,3-BISPHOSPHATIDYL-2-BENZOYL-SN-GLYCEROL DIMETHYL ESTER
ANALYSIS OF CARDIOLIPIN MOLECULAR-SPECIES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF ITS DERIVATIVE 1,3-BISPHOSPHATIDYL-2-BENZOYL-SN-GLYCEROL DIMETHYL ESTER
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DOI：
10.1016/0003-2697(91)90332-n
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发表时间：
1991-06-01
影响因子：
2.9
通讯作者：
OTTEN, D
中科院分区：
文献类型：
--
作者：
SCHLAME, M;OTTEN, D
心磷脂（CL, 1,3-二磷脂酰-sn-甘油）是一种四酰基链磷脂，其分子种类组成不能用标准方法分析。本文报道了用高效液相色谱法测定其衍生物1,3-二磷脂酰-2-苯甲酰-sn-甘油二甲酯的分子种类的方法。采用1h核磁共振、紫外光谱、薄层色谱、脂肪酸分析等方法对其进行了表征。衍生化过程没有改变脂肪酸谱，并提供了一个几乎完全的转化为高度极性，紫外可见的产品。在228 nm吸光度的HPLC分离中，发现单个峰的面积与它们的脂质磷含量呈线性相关。牛心CL被分解为11个分子种，其中6种（占97 mol%）可被鉴定。牛心脏CL分子种类的对数保留时间与其双键数呈线性关系。
Cardiolipin (CL, 1,3-bisphosphatidyl-sn-glycerol) is a four-acyl-chain phospholipid whose molecular species composition cannot be analyzed by standard procedures. Here we report a method to resolve the molecular species of CL by high-performance liquid chromatography of its derivative 1,3-bisphosphatidyl-2-benzoyl-sn-glycerol dimethyl ester. The CL derivative was characterized by1H nuclear magnetic resonance spectroscopy, ultraviolet (uv) spectroscopy, thin-layer chromatography, and fatty acid analysis. The derivatization procedure did not change the fatty acid profile and provided a virtually complete conversion to the highly apolar, uv-visible product. In HPLC separations, recorded by 228 nm absorbance, a linear correlation was found between the area of individual peaks and their amount of lipid phosphorus. Bovine heart CL was resolved into 11 molecular species of which 6 (together accounting for 97 mol%) could be identified. The molecular species of bovine heart CL feature a linear relationship between their logarithmic retention time and their double bond number.