Regulation of cholinergic expression in cultured spinal cord neurons.
Regulation of cholinergic expression in cultured spinal cord neurons.
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培养的脊髓神经元中胆碱能表达的调节。
DOI：
10.1016/0012-1606(90)90308-6
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发表时间：
1990
影响因子：
2.7
通讯作者：
Kessler,JA
中科院分区：
文献类型：
--
作者：
Lombard-Golly,D;Wong,V;Kessler,JA
在离体培养的胚胎大鼠脊髓中研究了胆碱能神经元发育的调控因素。胆碱乙酰转移酶（CAT）的活性水平在新鲜分离的细胞迅速下降，保持低的第一周在文化中，然后增加。增加细胞密度或用脊髓膜处理可以部分阻止酶活性的降低。CAT活性也刺激治疗与MANS，从脊髓膜溶解的分子。MANS的作用在低密度培养物和新鲜接种的细胞中最大，表明该分子可以替代高密度和细胞-细胞接触的作用。CAT活性在腹侧（运动神经元富集）脊髓文化同样调节密度升高或治疗MANS，而酶活性在这些条件下，在mediodorsal（自主神经元富集）文化基本不变。这些观察结果表明，胆碱能运动神经元和自主神经元的发育不受相同的因素。用MANS处理腹侧脊髓培养物并没有增加用单克隆CAT抗体免疫细胞化学检测到的胆碱能神经元的数量，这表明MANS并没有增加运动神经元的存活，而是刺激了每个神经元的CAT活性水平。这些观察结果表明，运动神经元的发育可以通过细胞-细胞接触来调节，MANS因子可能介导细胞-细胞接触对胆碱能表达的刺激作用。
Factors regulating development of cholinergic spinal neurons were examined in cultures of dissociated embryonic rat spinal cord. Levels of choline acetyltransferase (CAT) activity in freshly dissociated cells decreased rapidly, remained low for the first week in culture, and then increased. The decrease in enzyme activity was partially prevented by increased cell density or by treatment with spinal cord membranes. CAT activity was also stimulated by treatment with MANS, a molecule solubilized from spinal cord membranes. The effects of MANS were greatest in low-density cultures and in freshly plated cells, suggesting that the molecule may substitute for the effects of elevated density and cell-cell contact. CAT activity in ventral (motor neuron-enriched) spinal cord cultures was similarly regulated by elevated density or treatment with MANS, whereas enzyme activity was largely unchanged in mediodorsal (autonomic neuron-enriched) cultures under these conditions. These observations suggest that development of cholinergic motor neurons and autonomic neurons are not regulated by the same factors. Treatment of ventral spinal cord cultures with MANS did not increase the number of cholinergic neurons detected by immunocytochemistry with a monoclonal CAT antibody, suggesting that MANS did not increase motor neuron survival but rather stimulated levels of CAT activity per neuron. These observations indicate that development of motor neurons can be regulated by cell-cell contact and that the MANS factor may mediate the stimulatory effects of cell-cell contact on cholinergic expression.