Significance of hyphae formation in virulence of Candida tropicalis and transcriptomic analysis of hyphal cells
Significance of hyphae formation in virulence of Candida tropicalis and transcriptomic analysis of hyphal cells
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热带假丝酵母菌丝形成的毒力意义及菌丝细胞的转录组分析
DOI：
10.1016/j.micres.2016.06.003
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发表时间：
2016-11-01
影响因子：
6.7
通讯作者：
Peng, Yibing
中科院分区：
文献类型：
--
作者：
Jiang, Cen;Li, Zhen;Peng, Yibing
近期，热带念珠菌在临床分离株中的占比显著上升。部分热带念珠菌菌株作为共生菌定殖于皮肤或黏膜表面；另一些则引发侵袭性感染。截至目前，热带念珠菌的致病性尚未得到深入研究。本研究对从中国四个省份五家医院收集的52株热带念珠菌临床分离株的多种毒力因子进行了报道，这些毒力因子包括生物被膜与菌丝形成能力、蛋白酶、磷脂酶、脂肪酶以及溶血活性。在相同条件下，部分热带念珠菌比其他菌株更易于产生菌丝。将6株不同形态的热带念珠菌通过尾静脉注射到小鼠体内，对这些菌株导致的小鼠存活比例及真菌负荷进行评估。热带念珠菌产生菌丝的能力与其更强的毒力相关。RNA测序显示，产生更多菌丝的热带念珠菌上调了多个参与形态分化和氧化应激反应的基因，包括IF2、Abc1和Sod2。由此可见，菌丝形成在热带念珠菌的致病性中起着至关重要的作用，并且与氧化应激反应相互作用以增强该菌的毒力。（C）2016爱思唯尔股份有限公司。保留所有权利。
Recently, the proportion of Candida tropicalis in clinical isolates has significantly increased. Some C tropicalis strains colonize the skin or mucosal surfaces as commensals; others trigger invasive infection. To date, the pathogenicity of C. tropicalis has not been thoroughly researched. This study reports several virulence factors, including biofilm and hyphae formation, proteinase, phospholipase, lipase and hemolytic activity, in 52 clinical isolates of C. tropicalis collected from five hospitals in four provinces of China. Some C. tropicalis tended to produce more hyphae than others in the same circumstance. Six C. tropicalis strains with different morphologies were injected into mice via the tail vein, and the survival proportions and fungal burdens of the strains were evaluated. Hyphal production by C tropicalis was associated with stronger virulence. RNA sequencing revealed that C tropicalis with more hyphae up-regulated several genes involved in morphological differentiation and oxidative response, including IF2, Abc1, and Sod2. It appears that hyphal formation plays a vital role in the pathogenicity of C. tropicalis, and interacts with the oxidative stress response to strengthen the organism's virulence. (C) 2016 Elsevier GmbH. All rights reserved.