Encoding of conditioned fear in central amygdala inhibitory circuits
Encoding of conditioned fear in central amygdala inhibitory circuits
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DOI：
10.1038/nature09559
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发表时间：
2010-11-11
期刊：
影响因子：
64.8
通讯作者：
Luethi, Andreas
中科院分区：
文献类型：
--
作者：
Ciocchi, Stephane;Herry, Cyril;Luethi, Andreas
中央杏仁核（CEA）是一个主要由GABA能抑制性神经元组成的核团，在恐惧条件反射中起重要作用。条件性恐惧的获得和表达是如何在CEA抑制回路中编码的尚不清楚。在小鼠体内使用电生理学，光遗传学和药理学方法，我们表明，中央杏仁核（CE1）的外侧亚区的神经元活动是需要恐惧收购，而条件性恐惧反应是由内侧亚区（CEm）的输出神经元驱动。功能电路分析表明，抑制CEA微电路是高度组织化的，并且CEl至CEm通路中的阶段性和紧张性活动的细胞类型特异性可塑性可以门控恐惧表达并调节恐惧泛化。我们的研究结果定义了CEA微电路的功能结构及其在条件性恐惧行为的获得和调节中的作用。
The central amygdala (CEA), a nucleus predominantly composed of GABAergic inhibitory neurons, is essential for fear conditioning. How the acquisition and expression of conditioned fear are encoded within CEA inhibitory circuits is not understood. Using in vivo electrophysiological, optogenetic and pharmacological approaches in mice, we show that neuronal activity in the lateral subdivision of the central amygdala (CEl) is required for fear acquisition, whereas conditioned fear responses are driven by output neurons in the medial subdivision (CEm). Functional circuit analysis revealed that inhibitory CEA microcircuits are highly organized and that cell-type-specific plasticity of phasic and tonic activity in the CEl to CEm pathway may gate fear expression and regulate fear generalization. Our results define the functional architecture of CEA microcircuits and their role in the acquisition and regulation of conditioned fear behaviour.