Candidiasis in interferon-γ knockout (IFN-γ-/-) mice
Candidiasis in interferon-γ knockout (IFN-γ-/-) mice
复制标题
DOI：
10.1086/515645
复制
发表时间：
1998-08-01
影响因子：
6.4
通讯作者：
Warner, T
中科院分区：
文献类型：
--
作者：
Balish, E;Wagner, RD;Warner, T
无菌C57BL/6 × 129干扰素γ敲除（ifn - γ(-/-)）小鼠和免疫正常（+/-,+/+）小鼠用纯培养的白色念珠菌定植，以评估它们对内源性粘膜和全身念珠菌病的自然易感性。在为期15周的研究中，纯培养的白色念珠菌对成年或新生的ifn - γ(-/-)非生物小鼠没有致死作用。与免疫正常的对照组相比，ifn - γ(-/-)小鼠对胃（心-窦切面）、肛肠和急性全身（静脉注射）念珠菌病更敏感，一些ifn - γ(-/-)小鼠在感染白色念珠菌后发生肠腺瘤。与免疫正常对照相比，ifn - γ(-/-)小鼠的敏感性增强可能与脾脏细胞对白色念珠菌抗原的增殖反应和T辅助2 （IgG1）血清抗体对白色念珠菌抗原的反应较差有关。因此，ifn - γ对小鼠对胃、肛门直肠和急性全身念珠菌病的抗性很重要。
Germ-free C57BL/6 x 129 interferon-gamma knockout (IFN-gamma(-/-)) mice and their immunocompetent (+/-, +/+) counterparts were colonized with a pure culture of Candida albicans to assess their natural susceptibility to mucosal and systemic candidiasis of endogenous origin. Colonization with a pure culture of C. albicans was not lethal for adult or neonatal IFN-gamma(-/-) gnotobiotic mice over the 15-week study. The IFN-gamma(-/-) mice were more susceptible to gastric (cardia-antrum section), anorectal, and acute systemic (intravenous challenge) candidiasis than immunocompetent controls, and some IFN-gamma(-/-) mice developed intestinal adenomas after colonization with C. albicans. The enhanced susceptibility of IFN-gamma(-/-) mice, compared with immunocompetent controls, may be associated with a poor proliferative response of spleen cells to C. albicans antigens and a T helper 2 (IgG1) serum antibody response to C. albicans antigens. Thus, IFN-gamma is important for murine resistance to gastric, anorectal, and acute systemic candidiasis.