Inhibition of influenza virus infection by a novel antiviral peptide that targets viral attachment to cells
Inhibition of influenza virus infection by a novel antiviral peptide that targets viral attachment to cells
复制标题
DOI：
10.1128/jvi.01678-06
复制
发表时间：
2006-12-01
影响因子：
5.4
通讯作者：
Schultz-Cherry, Stacey
中科院分区：
文献类型：
--
作者：
Jones, Jeremy C.;Turpin, Elizabeth A.;Schultz-Cherry, Stacey
甲型流感病毒继续造成广泛的发病率和死亡率。另一个令人担忧的问题是，目前在世界许多地区发现的高致病性H5N1甲型流感病毒对公共卫生构成严重威胁，并可能导致大流行。遏制流感流行或大流行的干预战略是高度优先的，重点是疫苗和抗病毒药物。在这些研究中，我们证明了来自成纤维细胞生长因子4信号序列的20个氨基酸的多肽(EB，进入阻滞剂)在体外对包括H5N1亚型在内的流感病毒具有广谱的抗病毒活性。EB多肽在体内具有保护性，即使在感染后给药也是如此。从机制上讲，EB多肽抑制与细胞受体的附着，防止感染。进一步的研究表明，EB多肽与病毒血凝素蛋白特异性结合。这种新的多肽具有作为研究病毒附着的试剂和作为未来治疗药物的潜在价值。
Influenza A viruses continue to cause widespread morbidity and mortality. There is an added concern that the highly pathogenic H5N1 influenza A viruses, currently found throughout many parts of the world, represent a serious public health threat and may result in a pandemic. Intervention strategies to halt an influenza epidemic or pandemic are a high priority, with an emphasis on vaccines and antiviral drugs. In these studies, we demonstrate that a 20-amino-acid peptide (EB, for entry blocker) derived from the signal sequence of fibroblast growth factor 4 exhibits broad-spectrum antiviral activity against influenza viruses including the H5N1 subtype in vitro. The EB peptide was protective in vivo, even when administered postinfection. Mechanistically, the EB peptide inhibits the attachment to the cellular receptor, preventing infection. Further studies demonstrated that the EB peptide specifically binds to the viral hemagglutinin protein. This novel peptide has potential value as a reagent to study virus attachment and as a future therapeutic.