Expression of Human Adenosine Deaminase in Mice After Transplantation of Genetically-Modified Bone Marrow
Expression of Human Adenosine Deaminase in Mice After Transplantation of Genetically-Modified Bone Marrow
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转基因骨髓移植后小鼠体内人腺苷脱氨酶的表达
DOI：
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Á.
中科院分区：
文献类型：
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作者：
A. R.;Á.
用高滴度逆转录病毒载体将人腺苷脱氨酶（h-ADA）cDNA转染小鼠骨髓细胞。h-ADA cDNA连接到逆转录病毒启动子，载体还含有新霉素磷酸转移酶基因作为选择标记。将感染的骨髓移植到同基因的W/W'受体中，并监测h-ADA表达5.5个月。移植后几周，在所有9名受体的红细胞中检测到h-ADA，其中8名受体的表达水平与内源性酶相等。这种表达水平在六只存活小鼠中的两只中持续存在，而在另外三只小鼠中的表达稳定在较低但易于检测的水平。只有一只小鼠在5.5个月后没有检测到h-ADA。在5.5个月的小鼠造血和淋巴组织中发现了具有共同整合位点的载体DNA序列，这提供了造血干细胞在小鼠体内表达的证据。
A high titer retroviral vector was used to transfer a human adenosine deaminase (h-ADA) cDNA into murine bone marrow cells in vitro. The h-ADA cDNA was linked to the retroviral promoter, and the vector also contained a neomycin phosphotransferase gene as a selectable marker. Infected marrow was transplanted into syngeneic W/W’ recipients, and h-ADA expression was monitored for 5.5 months. Several weeks after transplantation, h-ADA was detected in the erythrocytes of all nine recipients, eight of which expressed levels equal to the endogenous enzyme. This level of expression persisted in two of six surviving mice, while expression in three others stabilized at lower, but readily detectable, levels. Only one mouse had no detectable h-ADA after 5.5 months. Vector DNA sequences with common integration sites were found in hematopoietic and lymphoid tissues of the mice at 5.5 months, providing evidence that hematopoietic stem cells
基因转移至人造血细胞系后，人腺苷脱氨酶从各种强启动子的表达。
影响因子：
20.3
作者：
Hock,RA;Miller,AD;Osborne,WR
通讯作者：
Osborne,WR
通过重组逆转录病毒转移至造血干细胞的基因的表达。
DOI：
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发表时间：
1987
期刊：
Molecular biology & medicine
影响因子：
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作者：
Bowtell,DD;Johnson,GR;Kelso,A;Cory,S
通讯作者：
Cory,S