Association of Polymorphisms in the Interleukin-18 Gene With Susceptibility to Biliary Atresia
Association of Polymorphisms in the Interleukin-18 Gene With Susceptibility to Biliary Atresia
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DOI：
10.1097/mpg.0b013e3182111b9b
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发表时间：
2011-05
影响因子：
2.9
通讯作者：
Hung-Chang Lee;Tzu-yang Chang;C. Yeung;W. Chan;Chuen-Bin Jiang;H. Chan;Wei-Fang Chen;Horng-Woei Yang;Marie Lin;Yann-Jinn Lee
中科院分区：
文献类型：
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作者：
Hung-Chang Lee;Tzu-yang Chang;C. Yeung;W. Chan;Chuen-Bin Jiang;H. Chan;Wei-Fang Chen;Horng-Woei Yang;Marie Lin;Yann-Jinn Lee
背景和目的：胆道闭锁是新生儿的一种破坏性炎症性闭塞性胆管疾病，可累及肝内和肝外胆管。虽然病因尚不清楚，但免疫介导的胆管损伤可能是由尚不确定的感染性因素引起的。白介素18(IL-18)是一种促炎细胞因子，可诱导干扰素-γ，在免疫性、感染性和炎症性疾病中发挥重要作用。在这项研究中，我们调查了IL18基因的多态性是否与BA的易感性有关。患者和方法：从50名患有BA的台湾儿童和1117名种族匹配的健康对照的全血样本中提取基因组DNA。采用TaqMan方法对IL18-1297T/C、-607C/A、-137G/C和+105A/C等位基因进行分型。结果：这些IL18基因变异体的基因型、等位基因、携带者和单倍型频率在BA患儿和健康儿童之间差异无统计学意义。结论：我们的数据表明IL18基因在台湾儿童的BA易感性中不起主要作用。
Background and Objective:Biliary atresia (BA) is a destructive inflammatory obliterative cholangiopathy of neonates that affects both intrahepatic and extrahepatic bile ducts. Although the etiology is unknown, immunologically mediated injury of the bile ducts triggered by as yet unidentified infectious agents is likely to play a critical role. Interleukin-18 (IL-18) is a proinflammatory cytokine that plays an important role in immune, infectious, and inflammatory diseases because of its induction of interferon-gamma. In this study, we investigated whether polymorphisms of the IL18 gene were associated with susceptibility to BA. Patients and Methods:Genomic DNA was extracted from whole-blood samples of 50 Taiwanese children with BA and 1117 ethnically matched healthy controls. The IL18 –1297 T/C, –607 C/A, –137 G/C, and +105 A/C polymorphisms were genotyped using the TaqMan assay. Results:No statistically significant differences of genotype, allele, carrier, and haplotype frequencies of these IL18 gene variants were found between children with BA and healthy controls. Conclusions:Our data suggest that the IL18 gene does not play a major role in BA predisposition in Taiwanese children.