The NDT80-like transcription factor CmNdt80a affects the conidial formation and germination, mycoparasitism, and cell wall integrity of Coniothyrium minitans
The NDT80-like transcription factor CmNdt80a affects the conidial formation and germination, mycoparasitism, and cell wall integrity of Coniothyrium minitans
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NDT80 样转录因子 CmNdt80a 影响 Coniothyrium minitans 的分生孢子形成和萌发、真菌寄生和细胞壁完整性
DOI：
10.1111/jam.15575
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发表时间：
2022-05-15
影响因子：
4
通讯作者：
Liu，Yong
中科院分区：
文献类型：
--
作者：
Yang，Xiaoxiang;Huang，Xiaoqin;Liu，Yong
目的NDT80是一种已知的转录因子，调节多种靶标，包括减数分裂、胁迫反应、丝状生长、性发育、生物膜形成、耐药性和毒力。但Ndt80类基因在不同真菌中的数量和作用存在很大差异。本研究旨在研究广为人知的菌核病生防剂微小拟青霉(Coniothyrium Minitans)中类NDT80基因的数量和功能。方法与结果通过对微小拟青霉基因组序列的检索，获得了两个编码NDT80类蛋白的基因CmNdt80a和CmNdt80b。RT-PCR分析表明，从菌丝生长阶段(48HPI)到分节成熟期(120HPI)，两个CmNdt80基因都在微小拟青霉的菌丝生长阶段(48HPI)到分节成熟期(120HPI)有结构性表达。通过基因敲除和互补实验，验证了CmNdt80a和CmNdt80b在微小隐翅虫中的作用。结果表明，ΔCmNdt80a突变体在马铃薯葡萄糖琼脂平板上表现出较浅的颜色和正常的生长速度。ΔCmNdt80a突变体对核盘菌产生分生孢子、寄生菌落和菌核的能力明显低于野生型和补充型。有趣的是，ΔCmNdt80a突变体对细胞壁应激源和细胞壁降解酶的敏感性较低，并且比野生型菌株和互补菌株的细胞壁更厚。但未发现CmNdt80b缺失的表型后果。结论在微小隐翅虫中存在两个类似Ndt80的基因CmNdt80a和CmNdt80b。CmNdt80a而不是CmNdt80b在分生孢子发生、真菌寄生和细胞壁完整性中起着至关重要的作用。本研究的意义和影响了解微小木霉分生孢子和寄生的调控机制有助于我们更有效地利用这种生防剂，提高我们对真菌生物学的认识。
AimsNDT80, a known transcriptional factor, regulates various targets, including regulation of meiosis, stress responses, filamentous growth, sexual development, biofilm formation, drug resistance, and virulence. But the numbers and roles of Ndt80‐like genes in different fungi are highly variable. This study aims to address the numbers and functions of Ndt80‐like genes in Coniothyrium minitans, a well‐known biocontrol agent against Sclerotinia diseases.Methods and ResultsHere, two genes (CmNdt80a and CmNdt80b) encoding NDT80‐like proteins were obtained by searching the genomic sequence of C. minitans. RT‐PCR analysis showed that both CmNdt80 genes were constitutively expressed in C. minitans from the hyphal growth stage (48 hpi) to the pycnidial maturation stage (120 hpi). The roles of CmNdt80a and CmNdt80b in C. minitans were verified through gene knockout and complementation experiments. The results showed that the ΔCmNdt80a mutants exhibited a lighter colour and normal growth rate on potato dextrose agar plates. The ability of the ΔCmNdt80a mutants to produce conidia and parasitize the colony and sclerotia of Sclerotinia sclerotiorum was markedly diminished compared with the wild‐type strain and complemented strain. Interestingly, the ΔCmNdt80a mutant showed less sensitivity to cell wall stressors and cell wall‐degrading enzymes, and had thicker cell walls than the wild‐type strain and complemented strain. However, no phenotypic consequences were discovered for the deletion of CmNdt80b.ConclusionsTwo Ndt80‐like genes, CmNdt80a and CmNdt80b, were present in C. minitans. CmNdt80a, but not CmNdt80b, plays a crucial role in conidiogenesis, mycoparasitism, and cell wall integrity.Significance and Impact of the StudyUnderstanding the mechanisms that regulate the conidiation and parasitism of C. minitans could help us to more efficiently utilize this biological control agent and advance our knowledge of fungal biology.