Evaluation of Metagenomic Deep Sequencing as a Diagnostic Test for Infectious Keratitis.
Evaluation of Metagenomic Deep Sequencing as a Diagnostic Test for Infectious Keratitis.
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评估宏基因组深度测序作为感染性角膜炎的诊断测试。
DOI：
10.1016/j.ophtha.2020.07.030
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发表时间：
2021-03
期刊：
影响因子：
13.7
通讯作者：
Doan T
中科院分区：
文献类型：
--
作者：
Lalitha P;Prajna NV;Sikha M;Gunasekaran R;Hinterwirth A;Worden L;Chen C;Zhong L;Liu Z;Lietman TM;Seitzman GD;Doan T
宏基因组深度测序 (MDS)，包括 RNA 测序 (RNA-seq) 和 DNA 测序 (DNA-seq)，具有提高诊断灵敏度和准确性的潜力3, 4。总 RNA/DNA 测序的公正性允许识别几乎所有病原体，并具有发现病原体的潜力。然而，这些深度测序技术在核酸回收、环境污染、正常微生物群和可变的生物信息学解释策略等方面面临挑战5。在缺乏直接观察的诊断感染性角膜炎的金标准的情况下，我们使用潜在类别分析（LCA）来评估传统诊断测试、RNA-seq和DNA-seq的敏感性和特异性。获得了机构审查委员会的批准。这项研究遵循《赫尔辛基宣言》的原则。所有患者均获得书面知情同意。
Metagenomic deep sequencing (MDS), both RNA sequencing (RNA-seq) and DNA sequencing (DNA-seq), carries potential for improved diagnostic sensitivity and accuracy3, 4. The unbiased nature of total RNA/DNA sequencing allows for identification of almost any pathogen and includes the potential for pathogen discovery. However, these deep sequencing techniques have challenges with nucleic acid recovery, contamination from the environment, normal microbiota, and variable bioinformatic interpretive strategies5.In the absence of a directly observed gold standard for the diagnosis of infectious keratitis, we used latent class analysis (LCA) to evaluate the sensitivity and specificity of conventional diagnostics tests, RNA-seq, and DNA-seq. Institutional Review Board approval was obtained. This study adhered to the tenets of the Declaration of Helsinki. Informed written consent was obtained from all patients.