Spectral near-infrared fluorescence imaging of curved surfaces using projection reconstruction algorithms.
Spectral near-infrared fluorescence imaging of curved surfaces using projection reconstruction algorithms.
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使用投影重建算法对曲面进行光谱近红外荧光成像。
DOI：
10.1002/cmmi.129
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发表时间：
2007
影响因子：
--
通讯作者：
Kobayashi,Hisataka
中科院分区：
文献类型：
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作者：
Hama,Yukihiro;Koyama,Yoshinori;Bernardo,Marcelino;Choyke,PeterL;Kobayashi,Hisataka
在体内光谱荧光成像已经使得可以非侵入性地可视化表面弯曲结构以及皮肤深处的结构。然而，由模糊产生的散焦已经成为创建解剖学上可解释的表面图像的障碍。在这里，我们提出了一种方法来纠正模糊诱导的弯曲结构在光谱荧光成像使用信号强度投影算法。在体模和动物模型中，其中在组织间注射具有近红外（NIR）范围内的发射光谱的量子点后对淋巴系统进行可视化，依次调整焦平面以获得包含从多个焦点采集的图像的z堆叠图像。将最大、最小、中值和平均强度投影应用于所得图像。使用体模，最小和中值强度投影图像表现出改善的去模糊，而在体内成像期间，中值强度投影图像比其他投影技术更清晰地可视化重要结构。图像叠加与随后应用适当的投影技术提供了一个简单的方法去模糊从曲面获得的活体光学图像，从而提高其解剖分辨率。版权所有© 2007约翰威利父子有限公司。
In vivospectral fluorescence imaging has made it possible to non‐invasively visualize superficial curved structures as well as structures deep to the skin. However, the defocus created by blurring has been an obstacle to creating anatomically interpretable surface images. Herein we present a methodology to correct for blurring induced by curved structures during spectral fluorescence imaging using signal intensity projection algorithms. In a phantom and an animal model in which the lymphatic system was visualized after the interstitial injection of quantum dots with emission spectra in the near‐infrared (NIR) range, the planes of focus were sequentially adjusted to obtain az‐stack of images which contains images acquired from multiple focal points. Maximum, minimum, median and average intensity projections were applied to the resulting images. Using the phantom, the minimum and the median intensity projection images demonstrated improved deblurring whereas duringin vivoimaging the median intensity projection images more clearly visualized important structures than did the other projection techniques. Image stacking with subsequent application of appropriate projection techniques provides a simple method for deblurringin vivooptical images obtained from curved surfaces, thus improving their anatomic resolution. Copyright © 2007 John Wiley & Sons, Ltd.