Overexpression of glutathione s-transferase alpha in clear cell renal cell carcinoma.
Overexpression of glutathione s-transferase alpha in clear cell renal cell carcinoma.
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DOI：
10.1309/aqxr-6b2q-pugd-638c
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发表时间：
2005-03
影响因子：
3.5
通讯作者：
S. Chuang;P. Chu;J. Sugimura;M. Tretiakova;V. Papavero;Kim L. Wang;M. Tan;F. Lin;B. Teh;Ximing J. Yang
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文献类型：
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作者：
S. Chuang;P. Chu;J. Sugimura;M. Tretiakova;V. Papavero;Kim L. Wang;M. Tan;F. Lin;B. Teh;Ximing J. Yang
为了确定其诊断价值，我们评估了谷胱甘肽S-转移酶α（GST-α）在大量肾细胞癌（RCC）中的表达。用含有21，632个cDNA克隆的互补DNA（cDNA）微阵列芯片分析了70例肾肿瘤的GST-α信使RNA（mRNA）水平。此外，348原发性肾肿瘤和24转移性RCC进行免疫组化分析与GST-α特异性抗体。与其他肾脏肿瘤（1/27 [3.7%]）相比，大多数透明细胞RCC（28/43 [65.1%]; 28/39 [71.8%]，经信息点调整）中GST-α mRNA显著升高（11.4倍）。在大多数透明细胞（166/202 [82.2%];平均强度，2.41）和转移性透明细胞RCC（17/24 [70.8%];平均强度，2.62）中证实了强和弥漫性GST-α免疫反应性。其他肾肿瘤类型未表现出显著的GST-α免疫反应性，证实了mRNA结果。通过cDNA微阵列和免疫组化分析，我们证明了GST-α作为透明细胞RCC的生物标志物。
To determine its diagnostic value, we evaluated glutathione S-transferase alpha (GST-alpha) expression in a large number of renal cell carcinomas (RCCs). GST-alpha messenger RNA (mRNA) levels from 70 renal neoplasms were analyzed with complementary DNA (cDNA) microarray chips containing 21,632 cDNA clones. Furthermore, 348 primary renal tumors and 24 metastatic RCCs were subjected to immunohistochemical analysis with a GST-alpha-specific antibody. GST-alpha mRNA was elevated significantly (11.4-fold) in a majority of clear cell RCCs (28/43 [65.1%]; 28/39 [71.8%] with adjustments for informative spots) compared with other kidney tumors (1/27 [3.7%]). Strong and diffuse GST-alpha immunoreactivity was demonstrated in a majority of clear cell (166/202 [82.2%]; mean intensity, 2.41) and metastatic clear cell RCCs (17/24 [70.8%]; mean intensity, 2.62). Other renal tumor types did not exhibit significant GST-alpha immunoreactivity, confirming mRNA results. Through cDNA microarrays and immunohistochemical analysis, we demonstrated GST-alpha as a biomarker for clear cell RCCs.