Inhibitory effect of procyanidin oligomer from elm cortex on the matrix metalloproteinases and proteases of periodontopahtogens
Inhibitory effect of procyanidin oligomer from elm cortex on the matrix metalloproteinases and proteases of periodontopahtogens
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DOI：
10.1034/j.1600-0765.2003.02604.x
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发表时间：
2003-06-01
影响因子：
3.5
通讯作者：
Kim, CK
中科院分区：
文献类型：
--
作者：
Song, SE;Choi, BK;Kim, CK
目的：研究榆皮部分纯化提取物（榆叶提取物）及其活性成分。原花青素低聚物（3至12个黄烷-3-醇单体，平均分子量为1518，平均聚合度为5.3）的混合物，可能对蛋白酶有抑制作用。背景：宿主来源的基质金属蛋白酶（MMPs）和细菌蛋白酶在牙周炎的牙龈组织破坏中起重要作用。这些蛋白酶的抑制剂可能发展成为治疗牙周炎的药物。方法：采用明胶酶谱法测定其抑菌效果。所测MMPs来源于成人牙周炎患者的龈沟液（GCF）和培养的牙周韧带（PDL）细胞的条件培养基。当用牙周病病原体处理时，提供proMMP-2并激活MMP-2。梅毒lecithinolyticum。检测的细菌酶是两种主要的牙周病病原体，牙龈卟啉单胞菌和牙密螺旋体的分泌形式。采用n-苯甲酰-精氨酸-萘酰胺（BANA）法测定两种牙周病病原菌对胰蛋白酶样酶的抑制作用。结果：榆木提取物和原花青素低聚物（100- 1000马克杯：ml）对GCF中MMPs（主要是MMP-8和MMP-9）有明显的抑制作用。MMP-2的前体和活性形式，以及2牙龈分泌的胰蛋白酶样酶。结论：榆皮可作为治疗牙周病的潜在药物。由于其对MMPs的抑制作用，终止了牙周病病原体的蛋白酶。
Objectives: The purpose of this study was to evaluate a partially purified extract (elm extract) troth the Ulmi cortex (Ulmi macrocarpa Hance) and its active ingredient. a mix of procyanidin oligomers (3 to 12 flavan-3-ol monomers, an average molecular weight of 1,518 with an average polymerization degree of 5.3) for a possible inhibitory effect against proteases.Background: Host-derived matrix metalloproteinases (MMPs) and bacterial protease,, play important roles in the gingival tissue destruction that is a characteristic of periodontitis. The inhibitors of these proteases may be developed into therapeutic agents against periodontitis.Methods: The inhibitory effects were assessed by gelatin zymography. The MMPs tested were originated from the gingival crevicular fluid (GCF) of adult periodontitis patients and from the conditioned media of cultured periodontal ligament (PDL) cells. which provided the proMMP-2 and activated MMP-2 when treated with a periodontopathogen. Treponema lecithinolyticum. Bacterial enzymes tested were secreted forms from two major periodontopathogens, Porphyromonas gingivalis and Treponema denticola. In addition, the inhibitory effects on trypsin-like enzymes from these two periodontopathogens were assayed by the n-benzoyl-(DL)-arginine-naphthylamide (BANA) test.Results: The elm extract and the procyanidin oligomer (100-1,000 mug:ml) exhibited potent inhibitory effects on the MMPs in GCF (chiefly MMP-8 and MMP-9). the pro and active forms of MMP-2, and secreted and trypsin-like enzymes from 2 gingivalis.Conclusions: These results suggest that elm cortex should be considered as a potential agent against periodontal diseases. due to its inhibitory action on MMPs end the proteases of periodontopathogens.