A role for MMS4 in the processing of recombination intermediates during meiosis in Saccharomyces cerevisiae.
A role for MMS4 in the processing of recombination intermediates during meiosis in Saccharomyces cerevisiae.
复制标题
DOI：
--
复制
发表时间：
2001-12
期刊：
影响因子：
3.3
通讯作者：
T. D. L. Santos;J. Loidl;B. Larkin;N. M. Hollingsworth
中科院分区：
文献类型：
--
作者：
T. D. L. Santos;J. Loidl;B. Larkin;N. M. Hollingsworth
酿酒酵母的MMS4基因最初是由于其在营养细胞中对MMS的敏感性而被鉴定的。随后的研究证实了MMS4在营养细胞的DNA代谢中的作用。此外，观察到mms4二倍体孢子形成不良。这项工作表明mms4孢子形成缺陷是由于减数分裂重组检查点的触发。遗传、物理和细胞学分析表明，MMS4在减数分裂重组的单端侵入步骤后发挥功能。在spo13二倍体，red1，而不是mek1，是上位性的孢子形成和孢子活力的MMS4，这表明MMS4可能只需要当同系物能够进行突触。MMS4和MUS81在孢子活力方面处于相同的上位性组，这与表明这两种蛋白质在复合物中起作用的生物化学数据一致。相反，MMS4在活孢子的产生中独立于MSH5发挥作用。我们建议，MMS4是需要在减数分裂过程中的特定重组中间体的加工。
The MMS4 gene of Saccharomyces cerevisiae was originally identified due to its sensitivity to MMS in vegetative cells. Subsequent studies have confirmed a role for MMS4 in DNA metabolism of vegetative cells. In addition, mms4 diploids were observed to sporulate poorly. This work demonstrates that the mms4 sporulation defect is due to triggering of the meiotic recombination checkpoint. Genetic, physical, and cytological analyses suggest that MMS4 functions after the single end invasion step of meiotic recombination. In spo13 diploids, red1, but not mek1, is epistatic to mms4 for sporulation and spore viability, suggesting that MMS4 may be required only when homologs are capable of undergoing synapsis. MMS4 and MUS81 are in the same epistasis group for spore viability, consistent with biochemical data that show that the two proteins function in a complex. In contrast, MMS4 functions independently of MSH5 in the production of viable spores. We propose that MMS4 is required for the processing of specific recombination intermediates during meiosis.