Nucleoside diphosphate kinase of halobacteria
Nucleoside diphosphate kinase of halobacteria
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盐细菌核苷二磷酸激酶
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
K. Horikoshi
中科院分区：
文献类型：
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作者：
Toru Mizuki;M. Kamekura;M. Ishibashi;R. Usami;Yasuhiko Yoshida;M. Tokunaga;K. Horikoshi
采用atp -琼脂糖层析法从12株盐杆菌中纯化核苷酸二磷酸激酶（NDK），并测定其n端氨基酸序列。当样品未经热处理时，这些酶在天然page和SDS-PAGE上的电泳迁移率有显著差异。haloarcula 7种与Har种7个氨基酸全序列的比较。加州和哈尔。目前尚未确定其n端3氨基酸的粳稻，在153个残基中，它们非常相似，只有1 ~ 4个残基不同。方藻和哈尔的NDKs。sinaiiensis仅在第30个氨基酸（精氨酸和半胱氨酸）上存在差异，但它们在盐响应模式上表现出显著差异，这表明单个氨基酸的取代可以导致最佳NaCl浓度的一个摩尔位移。
Nucleotide diphosphate kinase (NDK) was purified from 12 strains of halobacteria using ATP-agarose chromatography and their N-terminal amino acid sequences were determined. The electrophoretic mobilities of these enzymes differed significantly on the native-PAGE or the SDS-PAGE when the samples were not heat treated. Comparison of seven complete amino acid sequences from seven species of Haloarcula_and those from Har. californiae and Har. japonica, whose N-terminal 3 amino acids have not been determined yet, revealed that they are very similar and differed at only 1 to 4 residues in 153 residues. NDKs from Haloarcula quadrata and Har. sinaiiensis differed only at the 30th amino acid (arginine vs. cysteine), yet they showed a remarkable difference in their salt-response patterns, suggesting that a single amino acid substitution can cause a one molar shift in the optimal NaCl concentration.