Hyperbaric oxygenation prevented brain injury induced by hypoxia-ischemia in a neonatal rat model
Hyperbaric oxygenation prevented brain injury induced by hypoxia-ischemia in a neonatal rat model
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DOI：
10.1016/s0006-8993(02)03094-9
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发表时间：
2002-09-27
期刊：
影响因子：
2.9
通讯作者：
Zhang, JH
中科院分区：
文献类型：
--
作者：
Calvert, JW;Yin, W;Zhang, JH
在个体的早期胎儿或新生儿阶段期间缺氧缺血（HI）的发生导致未成熟神经元的损伤，从而导致行为和心理功能障碍，例如运动或学习障碍、脑瘫、癫痫甚至死亡。目前没有有效的治疗方法，这项研究是第一次使用高压氧（HBO）作为新生儿HI的治疗。在此，我们试图确定HBO是否能够提供针对HI损伤的神经保护作用。7日龄的幼鼠进行单侧颈动脉结扎，然后缺氧2.5小时（8%O-2，37 ℃）。HBO治疗是通过将幼鼠置于缺氧暴露后1小时的室中（3 ATA持续1小时）来进行的。根据同侧半球重量除以对侧半球重量、光学显微镜和EM评估脑损伤。缺氧暴露后5周进行感觉运动功能测试。HI后同侧大脑半球分别为52.65%和57.64%（P
The occurrence of hypoxia-ischemia (HI) during early fetal or neonatal stages of an individual leads to the damaging of immature neurons resulting in behavioral and psychological dysfunctions, such as motor or learning disabilities, cerebral palsy, epilepsy or even death. No effective treatment is currently available and this study is the first to use hyperbaric oxygen (HBO) as a treatment for neonatal HI. Herein, we sought out to determine if HBO is able to offer neuroprotectivity against an HI insult. Seven-day-old rat pups were subjected to unilateral carotid artery ligation followed by 2.5 h of hypoxia (8% O-2 at 37 degreesC). HBO treatment was administered by placing pups in a chamber (3 ATA for 1 h) 1 h after hypoxia exposure. Brain injury was assessed based on ipsilateral hemispheric weight divided by contralateral hemispheric weight, light microscopy, and EM. Sensorimotor functional tests were administered at 5 weeks after hypoxia exposure. After HI, the ipsilateral hemisphere was 52.65 and 57.64% (P