Role of nerve growth factor in modulation of gastric afferent neurons in the rat.
Role of nerve growth factor in modulation of gastric afferent neurons in the rat.
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DOI：
10.1152/ajpgi.00356.2002
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发表时间：
2003-03
期刊：
影响因子：
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通讯作者：
K. Bielefeldt;N. Ozaki;G. Gebhart
中科院分区：
文献类型：
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作者：
K. Bielefeldt;N. Ozaki;G. Gebhart
最近的研究表明，实验性溃疡与感觉神经元电压敏感性钠电流特性的变化有关。我们假设神经生长因子（NGF）有助于这些变化。通过向大鼠胃壁注射乙酸诱导胃溃疡。通过ELISA和免疫化学法测定NGF表达。通过向胃壁内注射逆行示踪剂标记感觉神经元。钠电流记录在胃感觉神经元从结状和背根神经节培养24小时，在存在的神经生长因子或中和神经生长因子抗体，分别。胃溃疡形成引起胃壁内NGF浓度升高和NGF免疫反应性增加。暴露于NGF引起TTX-抗性钠电流的显着增加，而TTX-敏感的钠电流保持不变。这与脊髓感觉神经元从失活中恢复的加速有关。在胃炎症过程中，胃壁中NGF的产生和释放可能有助于初级传入神经元的敏感化。
Recent studies demonstrated that experimental ulcers are associated with changes in the properties of voltage-sensitive sodium currents in sensory neurons. We hypothesized that nerve growth factor (NGF) contributes to these changes. Gastric ulcers were induced by acetic acid injection into the wall of the rat stomach. NGF expression was determined by ELISA and immunohistochemically. Sensory neurons were labeled by injection of a retrograde tracer into the gastric wall. Sodium currents were recorded in gastric sensory neurons from nodose and dorsal root ganglia cultured for 24 h in the presence of NGF or a neutralizing NGF antibody, respectively. Gastric ulcer formation caused a rise in NGF concentration within the gastric wall and an increase in NGF immunoreactivity. Exposure to NGF caused a significant increase in the TTX-resistant sodium current, whereas the TTX-sensitive sodium current remained unchanged. This was associated with an acceleration of the recovery from inactivation in spinal sensory neurons. Production and release of NGF in the gastric wall may contribute to sensitization of primary afferent neurons during gastric inflammation.