ABUNDANCE, POLYMORPHISM AND GENETIC-MAPPING OF MICROSATELLITES IN RICE
ABUNDANCE, POLYMORPHISM AND GENETIC-MAPPING OF MICROSATELLITES IN RICE
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DOI：
10.1007/bf00280220
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发表时间：
1993-10-01
期刊：
影响因子：
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通讯作者：
TANKSLEY, SD
中科院分区：
文献类型：
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作者：
WU, KS;TANKSLEY, SD
二核苷酸微卫星已被表征并用作水稻的遗传标记。使用poly(dG-dA).(dC-dT)和poly(dG-dT).(dC-dA)探针筛选水稻基因组文库表明，平均每225 kb出现一次(GA)n重复，每480 kb出现一次(GT)n重复。对随机选择的 10 个微卫星进行 DNA 测序表明，重复次数在 12 至 34 之间，水稻中的微卫星模式与人类和其他哺乳动物的微卫星模式相似。已经设计了这些微卫星位点以及四个已发表的含微卫星序列的引物，并用 20 个水稻品种检验了多态性程度。在 20 个水稻品种的所有微卫星位点上均观察到了 5 至 11 个等位基因。在一些微卫星位点中发现了籼稻和粳稻两个栽培亚种特有的等位基因。根据平行比较，所有微卫星标记的杂合度值均显着高于 RFLP 标记。十个微卫星位点已被遗传定位到四个水稻染色体上。水稻中微卫星的基因组分布似乎是随机的。
Dinucleotide microsatellites have been characterized and used as genetic markers in rice. Screening of a rice genomic library with poly(dG-dA).(dC-dT) and poly(dG-dT).(dC-dA) probes indicated that (GA)n repeats occurred, on average, once every 225 kb and (GT)n repeats once every 480 kb. DNA sequencing of ten randomly selected microsatellites indicated that the numbers of repeats ranged from 12 to 34 and that the patterns of microsatellites in rice were similar to those of humans and other mammals. Primers to these microsatellite loci as well as to four published microsatellite-containing sequences have been designed and degrees of polymorphism has been examined with 20 rice accessions. Multiple alleles, ranging from 5 to 11, have been observed at all the microsatellite loci in 20 rice accessions. Alleles specific to two cultivated subspecies, indica and japonica, were found in some microsatellite loci. Heterozygosity values of all the microsatellite markers were significantly higher than those of RFLP markers, based upon a parallel comparison. Ten microsatellite loci have been genetically mapped to four rice chromosomes. The genomic distribution of microsatellites appears to be random in rice.