Virulence in an insect model differs between mating types in Aspergillus fumigatus
Virulence in an insect model differs between mating types in Aspergillus fumigatus
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DOI：
10.3109/13693786.2010.512301
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发表时间：
2011-02-01
期刊：
影响因子：
2.9
通讯作者：
Christians, Julian K.
中科院分区：
文献类型：
--
作者：
Cheema, Manjinder S.;Christians, Julian K.
烟曲霉是一种机会致病真菌，最近被发现进行有性繁殖。以前的工作表明，交配类型之间的侵袭性不同，在本研究中，我们测试了体内模型中交配类型之间的毒力是否不同，即，蜡螟的幼虫。测定了20株A.烟曲霉分离株; 3株环境来源的MAT 1 -1分离株、5株临床来源的MAT 1 -1分离株、7株环境来源的MAT 1 -2分离株和5株临床来源的MAT 1 -2分离株。对于每种分离物，我们测量了六次重复的毒力，并且对于每次重复，分生孢子生长、收获并独立计数，并且将2，500个菌落形成单位注射到每10个G中。大蜡螟幼虫交配类型之间的毒力不同，与MAT 1 -1分离株注射幼虫的存活率较低。临床分离株和环境分离株的毒力也不同，但令人惊讶的是，注射环境分离株的幼虫存活率较低。鉴定毒力变异的潜在机制可能会发现治疗曲霉菌感染的新靶点。
Aspergillus fumigatus is an opportunistic fungal pathogen that has recently been found to undergo sexual reproduction. Previous work suggested that invasiveness differs between mating types, and in the present study we tested whether virulence differs between mating types in an in vivo model, i.e., larvae of the wax moth Galleria mellonella. We measured virulence of 20 A. fumigatus isolates; three MAT1-1 isolates of environmental origin, five MAT1-1 isolates of clinical origin, seven MAT1-2 isolates of environmental origin and five MAT1-2 isolates of clinical origin. For each isolate, we measured virulence in six replicates and for each replicate, conidia were grown, harvested, and counted independently, and 2,500 colony forming units were injected into each of 10 G. mellonella larvae. Virulence differed between mating types, with lower survival in larvae injected with MAT1-1 isolates. Virulence also differed between clinical and environmental isolates, but surprisingly larvae injected with environmental isolates had lower survival. Identification of the mechanisms underlying variation in virulence may identify novel targets for the treatment of Aspergillus infections.