SEQUENCE SPECIFICITY OF THE CORE-BINDING FACTOR
SEQUENCE SPECIFICITY OF THE CORE-BINDING FACTOR
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DOI：
10.1128/jvi.67.4.2408-2411.1993
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发表时间：
1993-04-01
影响因子：
5.4
通讯作者：
SPECK, NA
中科院分区：
文献类型：
--
作者：
MELNIKOVA, IN;CRUTE, BE;SPECK, NA
核心结合因子（CBF）结合哺乳动物C型逆转录病毒增强子中的保守核心基序。我们通过乙基化干扰试验分析了CBF对Moloney小鼠白血病病毒增强子的磷酸盐接触。磷酸盐接触跨越以共有核心位点为中心的9 bp。为了研究CBF结合的序列偏好，我们采用了选择和扩增结合序列足迹法（T。K. Blackwell和H. Weintraub，Science 250：1104-1110，1990）。通过选择和扩增的结合序列足迹法定义的CBF的共有结合位点是PyGPyG GTPy。
The core-binding factor (CBF) binds the conserved core motif in mammalian type C retrovirus enhancers. We analyzed the phosphate contacts made by CBF on the Moloney murine leukemia virus enhancer by ethylation interference assay. The phosphate contacts span 9 bp centered around the consensus core site. To examine the sequence preferences for CBF binding, we employed the technique of selected and amplified binding sequence footprinting (T. K. Blackwell and H. Weintraub, Science 250:1104-1110, 1990). The consensus binding site for CBF defined by selected and amplified binding sequence footprinting is PyGPyG GTPy.