Integration of danger peptide signals with HAMP signaling amplifies the anti-herbivore defense responses in rice
Integration of danger peptide signals with HAMP signaling amplifies the anti-herbivore defense responses in rice
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危险肽信号与 HAMP 信号的整合增强了水稻的抗草食动物防御反应
DOI：
10.1111/tpj.13883
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发表时间：
2018
期刊：
影响因子：
7.2
通讯作者：
Galis Ivan
中科院分区：
文献类型：
--
作者：
Shinya Tomonori;Yasuda Shigetaka;Hyodo Kiwamu;Tani Rena;Hojo Yuko;Fujiwara Yuka;Hiruma Kei;Ishizaki Takuma;Fujita Yasunari;Saijo Yusuke;Galis Ivan
植物对食草动物的防御由来自昆虫口腔分泌物（OS）和/或唾液的食草动物相关分子模式（HAMP）调节。此外，进食伤口启动植物自我损伤反应，这些反应由危险相关分子模式（DAMP）调节，如促进免疫防御的植物诱导肽（Peps）。虽然在食草动物中这两种模式的时间和空间共存意味着它们密切相互作用的可能性，但分子机制仍然不确定。在这里，我们报告，外源应用水稻（Oryza sativa）肽（OsPeps）可以引发水稻细胞培养物的多重防御反应。水稻叶片中OsPROPEP 3基因转录本被创伤和OS处理的特异性激活进一步表明OsPep 3肽可能参与水稻-植食性昆虫相互作用。相应地，我们发现同时应用OsPep 3和Mythimna loreyiOS显著放大了水稻细胞中的一系列防御反应，包括丝裂原活化蛋白激酶激活，以及防御相关激素和代谢产物的产生。OsPep 3对OsPROPEP 3/4的诱导作用表明OsPep信号传导中存在一个正向的自动反馈回路，这可能有助于防御信号的进一步增强。最后，OsPep受体OsPEPR 1的过表达增加了水稻植株不仅对同源OsPep而且对OS信号的敏感性。我们的研究结果共同表明，HAMP-DAMP信号整合在植物防御信号的放大提供了一个关键步骤。
Plant defense against herbivores is modulated by herbivore‐associated molecular patterns (HAMPs) from oral secretions (OS) and/or saliva of insects. Furthermore, feeding wounds initiate plant self‐damage responses modulated by danger‐associated molecular patterns (DAMPs) such as immune defense‐promoting plant elicitor peptides (Peps). While temporal and spatial co‐existence of both patterns during herbivory implies a possibility of their close interaction, the molecular mechanisms remain undetermined. Here we report that exogenous application of rice (Oryza sativa) peptides (OsPeps) can elicit multiple defense responses in rice cell cultures. Specific activation ofOsPROPEP3gene transcripts in rice leaves by wounding and OS treatments further suggests a possible involvement of the OsPep3 peptide in rice–herbivore interactions. Correspondingly, we found that simultaneous application of OsPep3 andMythimna loreyiOS significantly amplifies an array of defense responses in rice cells, including mitogen‐activated protein kinase activation, and generation of defense‐related hormones and metabolites. The induction ofOsPROPEP3/4by OsPep3 points to a positive auto‐feedback loop in OsPep signaling which may contribute to additional enhancement of defense signal(s). Finally, the overexpression of the OsPep receptor OsPEPR1 increases the sensitivity of rice plants not only to the cognate OsPeps but also to OS signals. Our findings collectively suggest that HAMP–DAMP signal integration provides a critical step in the amplification of defense signaling in plants.