A Simplified Method for separating Amino-acid Mixtures from Protein Hydrolysates on One Ion-Exchange Column during Preparation
A Simplified Method for separating Amino-acid Mixtures from Protein Hydrolysates on One Ion-Exchange Column during Preparation
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一种在制备过程中在一个离子交换柱上从蛋白质水解产物中分离氨基酸混合物的简化方法
DOI：
10.1038/1911198a0
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发表时间：
1961
期刊：
影响因子：
64.8
通讯作者：
M. Dobiášová
中科院分区：
文献类型：
--
作者：
J. Liebster;K. Kopoldová;M. Dobiášová
大多数用于分离氨基酸混合物的方法是基于Mooreet al.1 - 3的工作。在两个柱上实现分离，酸性氨基酸在"Dowex" 1阴离子交换柱上分离，碱性和中性氨基酸在"Dowex" 50阳离子交换柱上分离，或者通过在H-循环中在"Dowex" 50上的一个柱上进行色谱分离，用柠檬酸盐磷酸盐缓冲溶液进行洗脱。汉密尔顿和安德森4最近描述了一种半自动的方法，这些分离包含几个改进的原始过程。这些方法确保氨基酸的良好分离;然而，洗脱液含有氨基酸和盐酸的盐酸盐或大量难以除去的盐。分离氨基酸的有效方法应允许在短时间内以小体积的洗脱剂进行分离;所用缓冲液应可通过简单方法除去。Wilcoxet al.5使用挥发性缓冲液在阴离子交换柱上分离天冬氨酸和谷氨酸。Vanessačeket等人6和Tomášek 7成功地使用挥发性缓冲液在'Dowex' 50上分离肽。
MOST of the methods used for the separation of mixtures of amino-acids are based on the work of Mooreet al.1–3. Separation is achieved either on two columns, acidic amino-acids being separated on a ‘Dowex’ 1 anion-exchange column and the basic and neutral amino-acids on a ‘Dowex’ 50 cation-exchange column or by chromatography on one column on ‘Dowex’ 50 in the H-cycle, elution being carried out with citrate phosphate buffer solutions. Hamilton and Anderson4recently described a semi-automatic method for these separations containing several improvements of the original process. These methods ensure good separation of amino-acids; the eluates, however, contain either the hydrochlorides of the amino-acid and hydrochloric acid or considerable amounts of salts, which are difficult to remove. An efficient method for the separation of amino-acids should permit separation in a small volume of the elution reagent in a short time; the buffers used should be removable by a simple method. Wilcoxet al.5used volatile buffers for the separation of aspartic and glutamic acid on an anion-exchange column. Vaněčeket al.6and Tomášek7, used volatile buffers successfully for the separation of peptides on ‘Dowex’ 50.