Tissue Distribution of Menaquinone-7 and the Effect of α-Tocopherol Intake on Menaquinone-7 Concentration in Rats
Tissue Distribution of Menaquinone-7 and the Effect of α-Tocopherol Intake on Menaquinone-7 Concentration in Rats
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DOI：
10.3177/jnsv.64.391
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发表时间：
2018-01-01
影响因子：
1.6
通讯作者：
Uchida, Tomono
中科院分区：
文献类型：
--
作者：
Ikeda, Saiko;Hanzawa, Fumiaki;Uchida, Tomono
我们曾报道维生素E的摄入降低了大鼠肝外组织中叶醌(PK)的浓度。在本研究中，我们旨在通过与其他维生素K分布的比较，阐明发酵食品中维生素K的分布特点，并阐明维生素E摄入量对MK-7浓度的影响。分别饲喂无维生素K饲料(Free组)、含0.75 mg PK/kg饲料(PK组)、0.74 mg MK-4(MK-4)/kg饲料(MK-4组)、1.08 mg MK-7/kg饲料(MK-7组)、0.29 mg/kg甲萘二酮(MD)/kg饲料(MD组)，共16wk。PK-7组血清和所有组织中均有PK积聚，但MK-7组MK-7主要积聚在肝、脾和肾上腺。相反，在PK、MK-4、MK-7和MD组的所有组织中都有MK-4的表达。MK-4组血清、肝脏、脂肪组织和脾组织中MK-4浓度高于其他组，而肾、睾丸、胫骨和脑中MK-4浓度低于PK、MK-7和MD组。其次，给维生素E和K缺乏的大鼠灌胃给予MK-7加或不加α-生育酚，α-生育酚不影响血清和各种组织中MK-7或MK-4的浓度。这些结果表明，MK-7特别容易在肝脏中蓄积，并且MK-7的浓度不受维生素E摄入量的影响。
We have reported that vitamin E intake lowers phylloquinone (PK) concentration in extrahepatic tissues of rats. In this study, we aimed to clarify the characteristic of the distribution of menaquinone-7 (MK-7), a vitamin K contained in fermented foods, by comparison with other vitamin K distributions and to clarify the effect of vitamin E intake on MK-7 concentration in rats. Rats were fed a vitamin K-free diet (Free group), a diet containing 0.75 mg PK/kg (PK group), a 0.74 mg menaquinone-4 (MK-4)/kg diet (MK-4 group), a 1.08 mg MK-7/kg diet (MK-7 group), or a 0.29 mg menadione (MD)/kg diet (MD group) for 16 wk. MK-7 mainly accumulated in the liver, spleen, and adrenal gland of the MK-7 group, although PK accumulated in the serum and all tissues of the PK group. Conversely, MK-4 was present in all tissues of the PK, MK-4, MK-7, and MD groups. MK-4 concentration in the serum, liver, adipose tissue, and spleen was higher in the MK-4 group than in the other groups; however, MK-4 concentration in the kidney, testis, tibia, and brain was lower in the MK-4 group than in the PK, MK-7, and MD groups. Next, vitamin E-and K-deficient rats were orally administered MK-7 with or without alpha-tocopherol alpha-Tocopherol did not affect MK-7 or MK-4 concentration in the serum and various tissues. These results suggested that MK-7 is particularly liable to accumulate in the liver, and MK-7 concentration is not affected by vitamin E intake.