A 48-WELL MICRO CHEMOTAXIS ASSEMBLY FOR RAPID AND ACCURATE MEASUREMENT OF LEUKOCYTE MIGRATION
A 48-WELL MICRO CHEMOTAXIS ASSEMBLY FOR RAPID AND ACCURATE MEASUREMENT OF LEUKOCYTE MIGRATION
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DOI：
10.1016/0022-1759(80)90211-2
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发表时间：
1980-01-01
影响因子：
2.2
通讯作者：
LEONARD, EJ
中科院分区：
文献类型：
--
作者：
FALK, W;GOODWIN, RH;LEONARD, EJ
设计48孔趋化室以最小化较大的Blindwell或Boyden趋化室所需的操作时间和材料量。细胞和化学引诱物的剂量-反应曲线表明，结果与Blindwell室获得的结果相当或更好。每孔化学引诱物的体积为25 μ l;细胞数可低至10，000。设置该多孔单元和对膜过滤片进行染色所需的时间可以忽略不计。结合使用图像分析仪计数迁移细胞的数量，该方法适用于大群体患者中单核细胞功能状态的临床研究。
A 48-well chemotaxis chamber was designed to minimize manipulation time and amount of material required by the larger blindwell or Boyden chemotaxis chamber. Cell and chemoattractant dose-response curves showed that results were comparable to or better than those obtained with blindwell chambers. The volume of chemoattractant per well is 25 .mu.l; the number of cells can be as low as 10,000. The time needed for setting up this multiwell unit and for staining the membrane filter sheet is negligible. Combined with the use of an image analyzer to count the number of migrated cells, the method is suitable for clinical research on the functional state of monocytes in large groups of patients.