Low-magnitude mechanical signals that stimulate bone formation in the ovariectomized rat are dependent on the applied frequency but not on the strain magnitude
Low-magnitude mechanical signals that stimulate bone formation in the ovariectomized rat are dependent on the applied frequency but not on the strain magnitude
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DOI：
10.1016/j.jbiomech.2006.05.014
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发表时间：
2007-01-01
影响因子：
2.4
通讯作者：
Rubin, Clinton
中科院分区：
文献类型：
--
作者：
Judex, Stefan;Lei, Xin;Rubin, Clinton
越来越多的证据表明，如果以足够高的频率施加极小的机械信号，则可以作为骨组织的合成代谢信号。为了确定骨对低幅度高频参数的反应性是否受内分泌失衡的调节，将卵巢切除（OVX）的Sprague-Dawley大鼠以45 Hz（n = 6）或90 Hz（n = 6）进行10 min/天的全身振动（WBV，0.15 g），并与OVX年龄匹配的对照组（n = 6）进行比较。在体内使用另外5只大鼠，以确定诱导的骨表面应变幅度（和应变率）。在28天的协议，胫骨近端干骺端的骨形成率为159%，在90 Hz的大鼠相比，年龄匹配的对照组，但45 Hz的大鼠与对照组没有显着差异。90 Hz大鼠的骨形态表明，与对照组或45 Hz大鼠相比，股骨远端骨骺的骨小梁体积（22%和25%）和骨小梁厚度（11%和12%）显著增加。尽管骨骼对90 Hz信号的敏感性增强，但由该频率引起的应变幅度和应变率显著低于45 Hz振动期间，这表明基质应变以外的因素正在驱动合成代谢反应。理想情况下，这样的机械信号代表控制骨量和形态的非药理学手段，尽管骨吸收的全身压力。(c)2006爱思唯尔有限公司保留所有权利。
There is growing evidence that extremely small mechanical signals, if applied at a sufficiently high frequency, can serve as anabolic signals to bone tissue. To determine if the responsiveness of bone to low-magnitude, high-frequency parameters is modulated by endocrine imbalance, ovariectomized (OVX) Sprague-Dawley rats were subjected to whole body vibrations (WBV, 0.15 g) at 45 Hz (n = 6) or 90 Hz (n = 6) for 10 min/day, and compared to OVX age-matched controls (it = 6). Five additional rats were used, in vivo, to establish the induced bone surface strain magnitudes (and strain rates). Following a 28 d protocol, bone formation rates in the metaphysis of the proximal tibia were 159% greater in 90 Hz rats when compared to age-matched controls, but 45 Hz rats were not significantly different from controls. Bone morphology of 90 Hz rats indicated significant], greater trabecular bone volume (22% and 25%) and thicker trabeculae (11% and 12%) over either controls or 45 Hz rats in the epiphysis of the distal femur, respectively. Despite the enhanced sensitivity of the skeleton towards the 90 Hz signal, the strain magnitudes and strain rates induced by this frequency were significantly lower than during 45 Hz vibration, suggesting that factors other than matrix strain are driving the anabolic response. Ideally, such mechanical signals represent a non-pharmacologic means of controlling bone mass and morphology in spite of systemic pressures for bone resorption. (c) 2006 Elsevier Ltd. All rights reserved.