Fastidian gum:: the Xylella fastidiosa exopolysaccharide possibly involved in bacterial pathogenicity
Fastidian gum:: the Xylella fastidiosa exopolysaccharide possibly involved in bacterial pathogenicity
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DOI：
10.1111/j.1574-6968.2001.tb10836.x
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发表时间：
2001-09-25
影响因子：
2.1
通讯作者：
Arruda, P
中科院分区：
文献类型：
--
作者：
da Silva, FR;Vettore, AL;Arruda, P
革兰氏阴性细菌苛养木杆菌是第一个被完全测序的植物病原体。该物种会引起几种重要的经济植物病害，包括柑橘杂色失绿症（CVC）。对 X. fastidiosa 基因组序列的分析揭示了一个 12 kb DNA 片段，其中含有与野油菜黄单胞菌的胶操纵子密切相关的操纵子。参与糖前体合成的所有基因的存在，基因组中存在胞外多糖（EPS）生产调节因子。油菜黄原菌胶基因中的三个缺失表明，叶枯病菌能够合成与黄原胶不同的 EPS。这种新型 EPS 可能由聚合四糖重复单元组成，这些重复单元是通过在聚戊二烯醇磷酸酯载体上依次添加葡萄糖-1-磷酸、葡萄糖、甘露糖和葡萄糖醛酸而组装而成的。 (C) 2001 年欧洲微生物学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
The Gram-negative bacterium Xylella fastidiosa was the First plant pathogen to be completely sequenced. This species causes several economically important plant diseases, including citrus variegated chlorosis (CVC). Analysis of the genomic sequence of X. fastidiosa revealed a 12 kb DNA fragment containing an operon closely related to the gum operon of Xanthomonas campestris. The presence of all genes involved in the synthesis of sugar precursors, existence of exopolysaccharide (EPS) production regulators in the genome. and the absence of three of the X. campestris gum genes suggested that X. fastidiosa is able to synthesize an EPS different from that of xanthan gum. This novel EPS probably consists of polymerized tetrasaccharide repeating units assembled by the sequential addition of glucose-1-phosphate, glucose, mannose and glucuronic acid on a polyprenol phosphate carrier. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.