Effect of low androgen levels on IKca and SKca3 channels in rat penile corpus cavernosum
Effect of low androgen levels on IKca and SKca3 channels in rat penile corpus cavernosum
复制标题
低雄激素水平对大鼠阴茎海绵体IKca和SKca3通道的影响
作者：
Hu Zhao;Jun Jiang;J. Xia;R. Jiang
我们研究了低雄激素水平是否通过调节大鼠海绵体中中电导Ca 2+激活的K+通道（IKca）和小电导Ca 2+激活的K+通道3（SKca 3）的表达来影响勃起功能。36只健康雄性SD大鼠随机分为4周对照组、4周去势组、去势后4周雄激素替代组、8周对照组、8周去势组、去势后8周雄激素替代组。雄激素替代组去势后隔日皮下注射睾酮（3 mg/kg）。4周和8周后，测量最大海绵体内压/平均动脉压（ICPmax/MAP）。检测阴茎海绵体IKca、SKca 3、内皮型一氧化氮合酶（eNOS）和P-eNOS的表达。去势组ICPmax/MAP明显低于对照组和雄激素替代组（p < 0.01）。去势组IKca和SKca 3 mRNA表达明显低于对照组和雄激素替代组（p < 0.01）。去势组eNOS、P-eNOS、IKca和SKca 3蛋白表达较对照组和雄激素替代组显著降低（p < 0.01）。在低雄激素水平下，ICPmax/MAP可通过下调SKca 3和IKca的表达，抑制P-eNOS/eNOS，降低eNOS生物活性而降低。
We investigated whether low androgen levels affected erectile function by regulating the expressions of intermediate‐conductance Ca2+‐activated K+ channel (IKca) and small‐conductance Ca2+‐activated K+ channel 3 (SKca3) in corpus cavernous of rats. Thirty‐six healthy male SD rats were randomly divided into the 4‐week control group, 4‐week castration group, 4‐week androgen replacement after castration group, 8‐week control group, 8‐week castration group and 8‐week androgen replacement after castration group, respectively. The rats in the androgen replacement groups were subcutaneously injected with testosterone (3 mg/kg) every other day after castration. After 4 and 8 weeks, maximum intracavernous pressure/mean arterial pressure (ICPmax/MAP) was measured. Expressions of IKca, SKca3, endothelial nitric oxide synthase (eNOS) and P‐eNOS in penile corpus cavernosum were detected. ICPmax/MAP decreased significantly in the castration groups as compared to the control groups and the androgen replacement groups (p < 0.01). mRNA expressions of IKca and SKca3 decreased significantly in the castration groups as compared to the control groups and androgen replacement groups (p < 0.01). Protein expressions of eNOS, P‐eNOS, IKca and SKca3 in the castration groups were significantly reduced as compared to the control groups and androgen replacement groups (p < 0.01). Under low androgen levels, ICPmax/MAP can be reduced by down‐regulating the expressions of SKca3 and IKca, inhibiting P‐eNOS/eNOS and reducing eNOS bioactivity.
使用新型装置评估小鼠膀胱感觉。
影响因子：
2.1
作者：
Bicer,Fuat;Kim,JinYoung;Horowitz,Andrew;Daneshgari,Firouz;Liu,Guiming
通讯作者：
Liu,Guiming