The Chlamydomonas reinhardtii ODA3 gene encodes a protein of the outer dynein arm docking complex.
The Chlamydomonas reinhardtii ODA3 gene encodes a protein of the outer dynein arm docking complex.
复制标题
DOI：
10.1083/jcb.137.5.1069
复制
发表时间：
1997-06-02
期刊：
影响因子：
--
通讯作者：
Witman GB
中科院分区：
文献类型：
--
作者：
Koutoulis A;Pazour GJ;Wilkerson CG;Inaba K;Sheng H;Takada S;Witman GB
我们已经使用插入突变/基因标签技术来产生新的莱茵衣藻突变体，这些突变体在动力蛋白外臂的组装方面存在缺陷。在39个插入的odA突变体中，两个是先前未克隆的ODA3基因的等位基因，一个是未克隆的ODA10基因的等位基因，一个代表了一个新的oda基因(称为ODA12)。ODA3特别令人感兴趣，因为它对于将外动力蛋白臂和外动力蛋白臂对接复合体(oda-DC)组装到鞭毛双线微管(Takada，S.和R.Kamiya)上是必不可少的。1994年。J.细胞生物学。126：737-745)。以插入的DNA为标签，克隆了ODA3基因和全长cDNA。克隆的基因挽救了oda3突变体的表型。该序列预测了一种新的83.4-kD蛋白，具有广泛的卷曲结构域。Oda-DC含有三个多肽；氨基酸直接测序表明，这些多肽中最大的一个对应于ODA3。这种蛋白很可能在鞭毛轴丝上动力蛋白外臂的精确定位中发挥重要作用。
We have used an insertional mutagenesis/ gene tagging technique to generate new Chlamydomonas reinhardtii mutants that are defective in assembly of the outer dynein arm. Among 39 insertional oda mutants characterized, two are alleles of the previously uncloned ODA3 gene, one is an allele of the uncloned ODA10 gene, and one represents a novel ODA gene (termed ODA12). ODA3 is of particular interest because it is essential for assembly of both the outer dynein arm and the outer dynein arm docking complex (ODA-DC) onto flagellar doublet microtubules (Takada, S., and R. Kamiya. 1994. J. Cell Biol. 126:737– 745). Beginning with the inserted DNA as a tag, the ODA3 gene and a full-length cDNA were cloned. The cloned gene rescues the phenotype of oda3 mutants. The cDNA sequence predicts a novel 83.4-kD protein with extensive coiled-coil domains. The ODA-DC contains three polypeptides; direct amino acid sequencing indicates that the largest of these polypeptides corresponds to ODA3. This protein is likely to have an important role in the precise positioning of the outer dynein arms on the flagellar axoneme.