Compartmentation of aluminium in leaves of an Al-accumulator, Fagopyrum esculentum Moench.
Compartmentation of aluminium in leaves of an Al-accumulator, Fagopyrum esculentum Moench.
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DOI：
10.1007/s00425-002-0763-z
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发表时间：
2002-07-01
期刊：
影响因子：
4.3
通讯作者：
Iwashita, T
中科院分区：
文献类型：
--
作者：
Shen, RF;Ma, JF;Iwashita, T
荞麦（Fagopyrum esculentum Moench.）是一种富铝植物，但铝解毒的内部机制尚不完全清楚。我们通过直接分离原生质体和液泡研究了铝在这种植物（cv.Jianxi）叶子中的亚细胞定位。基于油光显微镜观察和细胞质和液泡标记酶的活性，获得了在铝溶液中水培的荞麦叶的纯原生质体和液泡。叶片中 80% 以上的铝存在于原生质体中。经 27 Al-核磁共振鉴定为草酸铝络合物（1:3比例）。原生质体中的草酸盐和铝定位于液泡中。这些结果表明，荞麦叶中铝的内部解毒是通过与草酸盐络合和隔离到液泡中实现的。
Buckwheat (Fagopyrum esculentum Moench.) is an Al-accumulating plant, but the internal mechanism(s) of detoxification of Al is not fully understood. We investigated the subcellular localization of Al in the leaves of this plant (cv. Jianxi) by directly isolating protoplasts and vacuoles. Pure protoplasts and vacuoles from the leaves of buckwheat, grown hydroponically in Al solution, were obtained based oil light-microscopic observation and the activities of marker enzymes of cytosol and vacuoles. More than 80% of total Al in the leaves was present in the protoplasts. and was identified as an Al-oxalate complex (1:3 ratio) by 27 Al-nuclear magnetic resonance. Oxalate and Al in the protoplasts was localized in the vacuoles. These results suggest that internal detoxification of Al in the buckwheat leaves is achieved by both complexation with oxalate and sequestration into vacuoles.