ISOLATION OF HIGHLY-ACTIVE PHOTOSYSTEM-II PARTICLES FROM A MUTANT OF CHLAMYDOMONAS-REINHARDTII
ISOLATION OF HIGHLY-ACTIVE PHOTOSYSTEM-II PARTICLES FROM A MUTANT OF CHLAMYDOMONAS-REINHARDTII
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DOI：
10.1111/j.1432-1033.1980.tb04894.x
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
WOLLMAN, FA
中科院分区：
文献类型：
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作者：
DINER, BA;WOLLMAN, FA
高活性的光系统-II颗粒使用洗涤剂快速分离，并从绿色拟南芥C的突变体中以良好的产率获得。莱因哈德氏菌这些粒子完全没有光系统I的反应中心，也没有光系统II的次级电子受体。它们显示出比活性（C550/单位叶绿素的Δ A）是起始材料和菠菜叶绿体的4-7倍;天线大小为40-50个叶绿素分子，含有很少的捕光叶绿素a/B复合物（叶绿素a/叶绿素B = 4-6.4）;可变荧光产率与暗适应荧光产率的比率高达3。通过离子交换色谱法对这些颗粒进行进一步处理，大大去除了5种蛋白质，并进一步减小了天线尺寸，而初级光活性几乎没有损失。
Highly active photosystem-II particles were rapidly isolated using detergents and obtained in good yield from a mutant of the green alga C. reinhardtii. The particles are completely devoid of reaction centers of photosystem I, and of the secondary electron acceptor to photosystem II. They show a specific activity (.DELTA.A of C550/unit chlorophyll) 4-7 times that of the starting material and of spinach chloroplasts; an antenna size of 40-50 chlorophyll molecules containing little light-harvesting chlorophyll a/b complex (chlorophyll a/chlorophyll b = 4-6.4); a ratio of variable to dark-adapted fluorescence yield of up to 3. Further treatment of these particles by ion-exchange chromatography largely removes 5 proteins and further decreases the antenna size with little loss in primary photoactivity.