Characterization and identification of Sox2+ radial glia cells derived from rat embryonic cerebral cortex
Characterization and identification of Sox2+ radial glia cells derived from rat embryonic cerebral cortex
复制标题
大鼠胚胎大脑皮层 Sox2 放射状胶质细胞的表征和鉴定
DOI：
10.1007/s00418-011-0864-5
复制
发表时间：
2011-11-01
影响因子：
2.3
通讯作者：
Zou, Linqing
中科院分区：
文献类型：
--
作者：
Li, Haoming;Jin, Guohua;Zou, Linqing
在中枢神经系统(CNS)发育过程中，放射状胶质细胞(RGC)至少扮演着两个重要的角色，它们参与神经元的产生和随后的神经元迁移的引导，但其在大脑皮层的确切分布和贡献尚不清楚。本研究以Vimentin为星形胶质细胞标记物，以Sox2为神经前体标记物，对胚胎16.5天大鼠大脑皮质RGCs进行鉴定和研究。我们发现Sox2+祖细胞定位于E16.5大脑皮质的生发区(GZ)，~95%的Sox2+细胞与Vimentin+或Nestin+放射状突起共存，这些突起穿过皮质板延伸到软脑膜表面。在体外，我们在贴壁条件下从E16.5大脑皮层获得了RG样细胞，这些Sox2+RG样细胞在体内具有RGCs的某些特性，并且这些Sox2+RG样细胞可以分化为星形胶质细胞、少突胶质细胞，并呈现出放射状胶质细胞-神经元的分化能力。综上所述，我们鉴定和研究了E16.5大脑皮层Sox2+RGCs的一些特性和特性，我们认为位于皮质GZ的胚胎Sox2+RGCs主要由Sox2+RGCs组成，皮质Sox2+RG样细胞在体外表现出放射状神经胶质细胞-神经元的分化能力。
During the central nervous system (CNS) development, radial glia cells (RGCs) play at least two essential roles, they contribute to neuronal production and the subsequent guidance of neuronal migration, whereas its precise distribution and contribution to cerebral cortex remains less understood. In this research, we used Vimentin as an astroglial marker and Sox2 as a neural progenitor marker to identify and investigate RGCs in rat cerebral cortex at embryonic day (E) 16.5. We found that the Sox2+ progenitor cells localized in the germinal zone (GZ) of E16.5 cerebral cortex, ~95% Sox2+ cells co-localized with Vimentin+ or Nestin+ radial processes which extended to the pial surface across the cortical plate (CP). In vitro, we obtained RG-like cells from E16.5 cerebral cortex on adherent conditions, these Sox2+ Radial glia (RG)-like cells shared some properties with RGCs in vivo, and these Sox2+ RG-like cells could differentiate into astrocytes, oligodendrocytes and presented the radial glia—neuron lineage differentiation ability. Taken together, we identified and investigated some characterizations and properties of Sox2+ RGCs derived from E16.5 cerebral cortex, we suggested that the embryonic Sox2+ progenitor cells which located in the cortical GZ were mainly composed of Sox2+ RGCs, and the cortex-derived Sox2+ RG-like cells displayed the radial glia—neuron lineage differentiation ability as neuronal progenitors in vitro.