Oral immunization with a replication-deficient recombinant vaccinia virus protects mice against influenza
Oral immunization with a replication-deficient recombinant vaccinia virus protects mice against influenza
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DOI：
10.1128/jvi.70.9.6418-6424.1996
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发表时间：
1996-09-01
影响因子：
5.4
通讯作者：
Small, PA
中科院分区：
文献类型：
--
作者：
Bender, BS;Rowe, CA;Small, PA
用两次灌胃给予剂量的含有来自H1N1流感病毒的血凝素和核蛋白基因的复制缺陷型重组牛痘病毒免疫的小鼠产生了血清抗H1免疫球蛋白G（IgG）抗体，其完全保护肺部免受H1N1的攻击。几乎所有给予两次灌胃剂量的小鼠也产生了粘膜抗H1伊加抗体，并且具有高抗H1伊加滴度的小鼠的鼻子完全受到保护。肌肉注射疫苗可以保护肺部，但不能保护鼻子免受攻击。我们还发现，该疫苗增强了由转变（H3N2）流感病毒引起的感染的恢复，可能是通过诱导核蛋白特异性细胞毒性T淋巴细胞活性。一种复制缺陷型口服肠溶牛痘病毒载体疫苗可能会安全地保护人类免受流感的侵害。
Mice immunized with two intragastrically administered doses of a replication deficient recombinant vaccinia virus containing the hemagglutinin and nucleoprotein genes from H1N1 influenza virus developed serum anti-H1 immunoglobulin G (IgG) antibody that completely protected the lungs from challenge with H1N1. Almost all of the mice given two intragastric doses also developed mucosal anti-H1 IgA antibody, and those with high anti-H1 IgA titers had completely protected noses. Intramuscular injection of the vaccine protected the lungs but not the noses from challenge. We also found that the vaccine enhanced recovery from infection caused by a shifted (H3N2) influenza virus, probably through the induction of nucleoprotein-specific cytotoxic T-lymphocyte activity. A replication-deficient, orally administered, enteric-coated, vaccinia virus-vectored vaccine might safely protect humans against influenza.