Microplastic particles are phagocytosed in gill cells of deep-sea and coastal mussels
Microplastic particles are phagocytosed in gill cells of deep-sea and coastal mussels
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DOI：
10.3389/fmars.2022.1034950
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发表时间：
2022-10
期刊：
影响因子：
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通讯作者：
T. Ikuta;Akihiro Tame;Tomoko Takahashi;H. Nomaki;R. Nakajima
中科院分区：
文献类型：
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作者：
T. Ikuta;Akihiro Tame;Tomoko Takahashi;H. Nomaki;R. Nakajima
越来越明显的是，全球海洋中的微塑料含量正在增加，这引发了人们对它们对海洋生态系统影响的担忧。微小的塑料颗粒不仅通过口服摄入进入海洋生物的体内，还通过体表（例如，鳃或表皮），但内化到细胞中的机制知之甚少。在这项研究中，我们使用深海化学合成贻贝进行了实验，通过将其鳃暴露于荧光标记的微塑料珠来限制摄食。我们确定了鳃细胞类型，优先内化珠，并证明了吞噬抑制剂对珠摄取的抑制作用。此外，使用相关的光电子显微镜，我们显微组织学验证，珠封闭在膜结合的空泡。我们的研究结果表明，微塑料颗粒内化到鳃细胞的深海和沿海贻贝的吞噬作用。这项研究强调，需要进一步研究通过身体表面的塑料污染，以保护高度地方性和脆弱的深海动物群，并减轻消费沿海双壳类动物对人类健康的风险。
It is becoming obvious that the abundance of microplastics is increasing in worldwide oceans, raising concerns about their impact on marine ecosystems. Tiny plastic particles enter the body of marine organisms not only via oral ingestion but also through the body surface (e.g., gills or epidermis), but the mechanism of internalization into cells is poorly understood. In this study, we conducted experiments using deep-sea chemosynthetic mussels with limited feeding by exposing their gills to fluorescently labeled microplastic beads. We identified the gill cell types that preferentially internalized the beads and demonstrated the inhibitory effect of phagocytosis inhibitors on bead uptake. Furthermore, using correlative light-electron microscopy, we microhistologically verified that beads were enclosed within membrane-bound vacuoles. Our results indicated that microplastic particles were internalized into gill cells of deep-sea and coastal mussels by phagocytosis. This study highlights the need for further research on plastic contamination via the body surface to conserve the highly endemic and vulnerable deep-sea fauna and mitigate human health risks from consuming coastal bivalves.