Antitumor and immunostimulatory activity of a polysaccharide-protein complex from Scolopendra subspinipes mutilans L. Koch in tumor-bearing mice
Antitumor and immunostimulatory activity of a polysaccharide-protein complex from Scolopendra subspinipes mutilans L. Koch in tumor-bearing mice
复制标题
蜈蚣多糖蛋白复合物对荷瘤小鼠的抗肿瘤和免疫刺激活性
DOI：
10.1016/j.fct.2012.05.018
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发表时间：
2012-08-01
影响因子：
4.3
通讯作者：
Yang, Jing
中科院分区：
文献类型：
--
作者：
Zhao, Haixia;Li, Ying;Yang, Jing
蜈蚣科赫在中国传统医学中用于癌症治疗已有数百年的历史。本研究观察了少刺蜈蚣多糖蛋白复合物（SPPC）对S180肉瘤和H22肝癌荷瘤小鼠肿瘤生长和免疫功能的影响。结果表明，SPPC能显著抑制小鼠移植瘤5180的生长，延长H22荷瘤小鼠的存活时间。对荷瘤小鼠的特异性和非特异性免疫应答有明显的促进作用，表现为NK细胞、CTL细胞活性和Th 1/Th 2细胞因子比值的提高，CD 4 + T细胞、B细胞和NK细胞的比例增加。此外，SPPC不仅显著抑制免疫抑制细胞因子（IL-10和TGF-β）的mRNA表达和产生，而且还减少了肿瘤相关巨噬细胞（TAM）中花生四烯酸（AA）代谢酶（考克斯-2和CYP 4A）及其产物（PGE（2）和20-HETE）。总之，我们的结果表明，SPPC通过至少部分地通过下调TAM中的AA代谢途径来改善抗肿瘤免疫应答来抑制体内肿瘤生长，并且可以作为具有免疫调节活性的抗肿瘤剂。(C)2012爱思唯尔有限公司保留所有权利。
Scolopendra subspinipes mutilans L. Koch has been used for cancer treatment in traditional Chinese medicine for hundreds of years. In this study, the effects of a polysaccharide-protein complex from Scolopendra subspinipes mutilans L Koch (SPPC) on the tumor growth and immune function were assessed in sarcoma S180 and hepatoma H22 bearing mice. Results showed that SPPC significantly inhibited the growth of 5180 transplanted in mice and prolonged the survival time of H22- bearing mice. In S180-bearing mice, it promoted specific and nonspecific immune response as evidenced by enhancing the activities of natural killer (NK) cells, cytotoxic T lymphocytes (CTL) and the ratio of Th1/Th2 cytokines, and increasing the percentages of CD4(+) T cells, B cells and NK cells. Furthermore, SPPC not only significantly inhibited mRNA expression and production of the immunosuppressive cytokines (IL-10 and TGF-beta), but also diminished arachidonic acid (AA)-metabolizing enzymes (COX-2 and CYP4A) and their products (PGE(2) and 20-HETE) in tumor-associated macrophages (TAMs). Taken together, our results indicate that SPPC inhibits tumor growth in vivo by improving antitumor immune responses at least partly via downregulating AA-metabolic pathways in TAMs, and could act as an anti-tumor agent with immunomodulatory activity. (C) 2012 Elsevier Ltd. All rights reserved.