Application of semipermeable membrane device (SPMD) to assess air genotoxicity in an occupational environment
Application of semipermeable membrane device (SPMD) to assess air genotoxicity in an occupational environment
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DOI：
10.1016/j.chemosphere.2009.02.039
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发表时间：
2009-06-01
期刊：
影响因子：
8.8
通讯作者：
Gilli, G.
中科院分区：
文献类型：
--
作者：
Bonetta, Sa;Carraro, E.;Gilli, G.
半透膜装置(SPMD)是一种被动采样器，可以隔离亲脂污染物，模拟生物脂肪组织中的生物浓缩。这项研究旨在评估SPMD和生物试验(彗星试验和Ames试验)在空气监测中的使用。为此，选择了预期存在多环芳烃(PAHs)污染的职业环境(焦化厂)作为案例研究。在五个职业性污染场所和一个对照场所部署了SPMD。对sPMD透析液进行化学分析，并用彗星试验检测其对人细胞(Jurkat)的DNA损伤活性，用Ames试验(TA98菌株，w/o S9)检测其诱变性。还收集并分析了总悬浮颗粒物(GC-MS)。对照部位未见SPMD提取物的生物学效应。另一方面，焦化厂内用SPMD收集的空气样本显示出不同程度的遗传毒性和诱变活性。结果支持了与SPMD采样相关的生物测试的敏感性，以评估潜在污染的工作环境的健康风险，强调了SPMD在环境空气质量监测中的有效性。(C)2009爱思唯尔有限公司。保留所有权利。
Semipermeable membrane device (SPMD) is a passive sampler that sequesters lipophilic contaminants, mimicking the bioconcentration in the fatty tissue of organisms. This study was designed to assess the use of SPMD and biological tests (Comet assay and Ames test) for air monitoring. For this purpose an occupational environment with expected polycyclic aromatic hydrocarbons (PAHs) contamination (coke plant) was selected for a case study. The SPMDs were deployed in five occupational contaminated sites and in a control site. The SPMD dialysates were chemically analysed and examined for in vitro DNA-damaging activity in human cells (Jurkat) by Comet assay and for mutagenicity with the Ames test (TA98 strain, w/o S9). Total suspended particulates were also collected and analysed (GC-MS). No biological effect of SPMD extract was revealed in the control site. On the other hand, air samples collected with SPMDs within the coke plant showed variable degrees of genotoxic and mutagenic activity. The highest effects were associated with the highest PAH level recovered in the SPMDs extracts and in particulate samples.Results obtained support the sensitivity of biological tests associated to SPMD sampling for evaluating the health risk of potentially contaminated work environments highlighting the usefulness of SPMDs for environmental air quality monitoring. (C) 2009 Elsevier Ltd. All rights reserved.