Comparison of TTC and Electrical Conductivity Heat Tolerance Screening Techniques in Phaseolus
Comparison of TTC and Electrical Conductivity Heat Tolerance Screening Techniques in Phaseolus
复制标题
菜豆TTC与电导率耐热性筛选技术比较
作者：
D. Schaff;C. D. Clayberg;G. Milliken
以26个菜豆品种为试材，比较了电导率和2，3，5-三苯基四氮唑还原试验两种估计热应激反应的基因差异的实验室技术。之前评估过耐热性。植物经过热驯化后，叶盘在一定的温度范围内受到热胁迫。通过对数据进行S型模型拟合，估计了在对照以上造成50%伤害的温度，即死亡温度。虽然品种的致死温度在试验之间存在相关性，但只有电导率试验的致死温度与田间胁迫下的产量表现相关。
Two laboratory techniques for estimating genotypic differences in response to heat stress—the electrical conductivity and the 2,3,5-triphenyl tetrazolium chloride reduction tests—were compared in tests with 26 cultivars of beans (Phaseolus vulgaris L.) previously evaluated for heat tolerance. After heat acclimation of plants, leaf disks were subjected to heat stress over a range of temperatures. The temperature causing 50% injury above the control, considered as the killing temperature, was estimated by fitting the data to a sigmoidal model. Although cultivar killing temperatures were correlated between tests, only killing temperatures for the conductivity test were correlated with yield performance under stress in the field.