An analysis of clinical disease activity and nephritis-associated serum autoantibody profiles in patients with systemic lupus erythematosus: a cross-sectional study
An analysis of clinical disease activity and nephritis-associated serum autoantibody profiles in patients with systemic lupus erythematosus: a cross-sectional study
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DOI：
10.1093/rheumatology/40.12.1405
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发表时间：
2001-12-01
期刊：
影响因子：
5.5
通讯作者：
Isenberg, DA
中科院分区：
文献类型：
--
作者：
Ravirajan, CT;Rowse, L;Isenberg, DA
客观的。使用系统性红斑狼疮 (SLE) 患者的横断面数据，建立狼疮性肾炎相关自身抗体水平与狼疮性肾炎的存在/活动性和整体疾病活动性之间的相关性。方法。使用不列颠群岛狼疮评估组 (BILAG) 指数评估疾病活动性。通过 ELISA 分析患有肾炎 (n=11) 和不患有肾炎 (n=22) 的 SLE 患者以及正常对照 (n=21) 中针对单链 DNA (ssDNA)、双链 DNA (dsDNA)、组蛋白、核小体和硫酸乙酰肝素 (HS) 的抗体水平。还分析了抗体亚类。结果。狼疮性肾炎患者的抗 dsDNA 和抗 HS 抗体水平较高，抗 HS 抗体水平与 BILAG 肾脏评分相关。 dsDNA 抗体的主要亚类是 IgG1 和 IgG3，抗核小体抗体的主要亚类是 IgG2，抗 HS 抗体的主要亚类是 IgG2 和 IgG3。结论。 dsDNA、ssDNA、组蛋白、核小体和 HS 抗体之间存在相关性。根据 BILAG 测量，狼疮性肾炎患者的抗 HS 抗体水平与疾病活动度之间存在很强的相关性。
Objective. To establish the correlation between lupus nephritis-associated autoantibody levels and the presence/activity of lupus nephritis and global disease activity using cross-sectional data in patients with systemic lupus erythematosus (SLE).Methods. Disease activity was assessed using the British Isles Lupus Assessment Group (BILAG) index. Antibody levels against single-stranded DNA (ssDNA), double-stranded DNA (dsDNA), histones, nucleosomes and heparan sulphate (HS) were analysed by ELISA in SLE patients with (n=11) and without (n=22) nephritis and in normal controls (n=21). Antibody subclasses were also analysed.Results. Higher levels of anti-dsDNA and anti-HS antibodies were found in patients with lupus nephritis, the level of anti-HS antibodies correlating with the BILAG renal score. Predominant subclasses were IgG1 and IgG3 for dsDNA antibodies, IgG2 for anti-nucleosome antibodies, and IgG2 and IgG3 for anti-HS antibodies.Conclusion. Correlation was demonstrated between antibodies to dsDNA, ssDNA, histones, nucleosomes and HS. There is a strong correlation between the level of anti-HS antibodies and disease activity in patients with lupus nephritis as measured by BILAG.