Measurement of the total proANP product in mammals by processing independent analysis
Measurement of the total proANP product in mammals by processing independent analysis
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DOI：
10.1016/j.jim.2011.06.005
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发表时间：
2011-07-29
影响因子：
2.2
通讯作者：
Goetze, Jens P.
中科院分区：
文献类型：
--
作者：
Hunter, Ingrid;Rehfeld, Jens F.;Goetze, Jens P.
在心脏病中，利钠肽前体的细胞加工减弱，导致释放未加工的前体、部分加工的片段和生物活性激素的混合物。在这里，我们报告了一种不依赖于物种的方法，用于定量心房利钠肽前体（proANP）及其产物，而不考虑可变的翻译后加工。开发了不依赖于加工的测定（PIA），其产生针对proANP中序列1-16的C末端的单特异性抗体。测定程序包括血浆提取，然后胰蛋白酶裂解，其从N-末端区域释放测定表位。在有心力衰竭症状的老年患者（n = 450）、急性心肌梗死猪（n = 21）、正常犬和心力衰竭犬（n = 77）中测试PIA。表位特异性允许在人、狗、猫和猪中进行可靠的测量。在人血浆中，PIA与已建立的proANP分析具有良好的相关性（r = 0.86，P
The cellular processing of natriuretic propeptides is attenuated in heart disease, resulting in release of a mixture of unprocessed precursor, partially processed fragments, and the bioactive hormone. Here, we report a species-independent method for quantification of pro-atrial natriuretic peptide (proANP) and its products irrespective of variable post-translational processing. The processing-independent assay (PIA) was developed raising mono-specific antibodies against the C-terminus of sequence 1-16 in proANP. The assay procedure included plasma extraction followed by tryptic cleavage, which releases the assay epitope from the N-terminal region. The PIA was tested in elderly patients with symptoms of heart failure (n = 450), in pigs with acute myocardial infarction (n = 21), and in normal dogs and dogs with heart failure (n = 77). The epitope specificity permitted reliable measurement in man, dog, cat and pig. In human plasma, the PIA correlated well with an established proANP analysis (r = 0.86, P