Concentration effect of prostaglandin E2 on the growth factor expression and cell proliferation in bovine endometrial explants and their kinetic characteristics
Concentration effect of prostaglandin E2 on the growth factor expression and cell proliferation in bovine endometrial explants and their kinetic characteristics
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前列腺素E2浓度对牛子宫内膜外植体生长因子表达和细胞增殖的影响及其动力学特性
DOI：
10.1111/rda.13083
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
J Cao
中科院分区：
文献类型：
--
作者：
S Zhang;W Mao;Q Li;R Gao;Y Zhang;L Gao;C Fu;J Wu;Y Deng;Y Shen;T Li;B Liu;J Cao
牛的子宫内膜在发情周期中经历各种生理和组织学变化，这些变化是胚泡着床所必需的。从发情前期到发情后期，子宫内膜逐渐增厚，为着床做好准备，并在子宫内膜脱落后表现出明显的自我修复能力，但未发生着床。在牛子宫内膜的发情周期中，前列腺素E2（PGE 2）的分泌模式与子宫内膜的生长同步;然而，关于PGE 2分泌与子宫内膜生长之间的相关性的信息有限。在这项研究中，浓度PGE 2处理对子宫内膜生长所必需的一系列生长因子的时间效应（2-36小时）和效应（10 - 9至10 - 5 M），包括结缔组织生长因子（CTGF）、成纤维细胞生长因子-2（FGF-2）、白细胞介素-8（IL-8）、转化生长因子-β1（TGF-β1）、基质金属蛋白酶-2（MMP-2）、体外培养牛子宫内膜组织，观察其血管内皮生长因子A（VEGFA）mRNA和蛋白表达及上皮细胞和成纤维细胞增殖情况。结果表明，10−7 ~ 10−5 M浓度的PGE 2可上调体外培养牛子宫内膜组织中CTGF、FGF-2、IL-8、MMP-2、TGF-β1、VEGFA mRNA和蛋白的表达，诱导上皮细胞和成纤维细胞增殖，并降低促凋亡因子caspase-3的表达。这些结果共同提高了PGE 2在发情周期中子宫内膜生长中的功能的可能性。
Bovine endometrium undergoes various physiological and histological changes that are necessary for blastocyst implantation during oestrous cycle. From pro-oestrus to late-oestrus, endometrium thickens gradually for implantation preparation and exhibits remarkable capacity for self-repair after uterine lining shedding while mplantation does not occur. The prostaglandin E2 (PGE2) secretion pattern is synchronized with endometrial growth during oestrous cycles in bovine endometrium; however, limited information is available regarding the association between PGE2 secretion and endometrial growth. In this study, the concentration (10−9 to 10−5 M) and time effect(2–36 hr) of PGE2 treatment on a series of growth factors are essential for endometrial growth including connective tissue growth factor (CTGF), fibroblast growth factor-2 (FGF-2),interleukin-8(IL-8),transforming growth factor-β1 (TGF-β1), matrix metalloproteinase-2(MMP-2),and vascular endothelial growth factor A (VEGFA) mRNA and protein expression, and proliferation of epithelial and fibroblast cells was investigated in bovine endometrial explants in vitro. The results indicated that PGE2 at concentration about 10−7 to 10−5 M could up-regulate CTGF, FGF-2,IL-8,MMP-2,TGF-β1,VEGFA mRNA and protein expression, and could induce the proliferation of epithelial and fibroblast cells and reduce the proapoptotic factor (caspase-3) expression in bovine endometrial explants in vitro. These results collectively improved the possibility of PGE2 functions in endometrial growth during oestrous cycles.